Binding of Isaac: Rebirth Wiki
Binding of Isaac: Rebirth Wiki

Added in Afterbirth

The Stain is a boss added in The Binding of Isaac: Afterbirth that can appear:


The Stain burrows underground when Isaac is not within range. While burrowed it becomes invincible and follows Isaac's movements. When close to Isaac, it pops out and performs one of the following attacks:

  • Sends out 2 patterns of numerous projectiles in all directions. The first barrage consists of fast-moving bullets that slow down. The second barrage consists of slow-moving bullets that speed up.
  • Sends out tendrils to its left and right leaving small puddles of damaging Red Creep directly around The Stain and to its either side.
  • Spawns 2 Chargers and can have a maximum of 3.

It will stay vulnerable for a brief moment after performing an attack before quickly burrowing underground again.

Champion Versions Needs to be unlocked[]

  • Added in Afterbirth † Grey: Continuously spawns 2 Round Worms instead of Chargers, with a maximum of 3 at any time. Never fires barrages of bullets.



When fighting The Stain following obstacles can sometimes spawn:

  • 2 red poops (near upper-right and bottom-left corners)
  • 2 red poops (near the center)
  • 4 red poops (in all corners)
  • 2x2 Pits in upper-right and bottom-left corners


  • It is the posthumous version of Polycephalus. It appears that the top heads have been stripped of their skin, while the bottom head only lacks its first skin layer.
    • Unlike its alive counterpart, the lower head has closed eyes and eyelids.
  • Added in Repentance The Stain has an alternative more degraded variation known as The Pile.
  • 2 Stains can spawn for the nightmare wave for the 6th floor of Greed Mode.
  • A third head can be seen in the background in The Stain's boss art.
  • During the versus screen, The Stain's popping out sound can be heard. The Stain is one of seven bosses that can be heard in the versus screen; the other six are The Forsaken, Added in Afterbirth † Big Horn, Mom, Mom's Heart (pre-Repentance), It Lives (pre-Repentance), and Added in Repentance Mother.
  • Prior to Booster Pack #5, The Stain could spawn Drowned Chargers if it was fought in the Flooded Caves. This also applied to Chub.

Bosses Boss Monstro.png
Chapter 1
Stage Basement icon.pngStage Cellar icon.pngStage Burning Basement icon.png
GeminiStevenBlighted OvumDingleThe Duke of FliesFamineGurglingsThe HauntLarry Jr.MonstroPinWidowThe FallenThe Headless Horseman
DangleLittle HornRag ManTurdlings
Baby Plum
Chapter 1.5
Stage Downpour icon.pngStage Dross icon.png
Lil BlubWormwoodThe RainmakerMin-MinClogColostomiaTurdlet
Chapter 2
Stage Caves icon.pngStage Catacombs icon.pngStage Flooded Caves icon.png
FistulaChubC.H.A.D.Carrion QueenDark OneGurdyGurdy Jr.The HollowThe HuskMega FattyMega MawPeepPestilencePolycephalusThe WretchedThe FallenThe Headless Horseman
The ForsakenThe FrailThe Stain
Big HornRag Mega
Chapter 2.5
Stage Mines icon.pngStage Ashpit icon.png
Reap CreepTuff TwinsHornfelGreat GideonSingeThe ShellThe Pile
Chapter 3
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The AdversaryThe BloatThe CageThe GateMonstro IIGishLokiMask of InfamyWarThe FallenThe Headless HorsemanMom
Sisters Vis
Reap CreepThe Pile
Chapter 3.5
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Chapter 4
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BlastocystThe BloatConquestDaddy Long LegsTriachnidDeathLokiiMama GurdyMr. FredScolexTeratomaThe FallenThe Headless HorsemanMom's HeartIt Lives
Sisters VisThe Matriarch
Stage Corpse icon.png
ChimeraThe ScourgeRotgutMother
Chapter 5
Stage Sheol icon.pngStage Cathedral icon.png
Chapter 6
Stage Dark Room icon.pngStage Chest icon.png
???The LambMega Satan
The Void
Stage The Void icon.png
Stage Home icon.png
DogmaThe BeastUltra DeathUltra FamineUltra PestilenceUltra War
Greed Mode
Ultra Greed
Ultra Greed
Ultra Greedier
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