The music in The Binding of Isaac: Rebirth, The Binding of Isaac: Afterbirth, The Binding of Isaac: Afterbirth † and The Binding of Isaac: Repentance was created by Ridiculon. The full albums for each consist of 32, 9, 4 and 31 tracks, respectively. However, Jesus Loves Uke, He’s the Number One, and Descensum are exclusive to the Rebirth album and thus not heard in-game, though Jesus Loves Uke can be seen unused in the resources. That being said, the Rebirth album doesn’t include the tracks for the intro and epilogue, adding back up to 31 in-game tracks. Afterbirth replaces Genesis with its own rendition titled Regenesis, resulting in a net gain of 8 tracks for a total of 39 tracks in Afterbirth. Afterbirth+ also has the Ante Lucem bonus track that cannot be heard in-game, so with a gain of 3 tracks there is a total of 42 tracks in Afterbirth+ that can be heard in-game. The Rebirth soundtrack can be purchased through Steam, however the Afterbirth, Afterbirth † and Repentance soundtracks cannot. All three soundtracks are purchasable from Ridiculon's Bandcamp page.
Nearly every main floor track gains instruments when enough smaller enemies or bosses are in the room. The variant can be heard in the soundtrack version in the second loop of the song. What instruments gained are indicated in bold next to the tracks.
The Binding of Isaac: Rebirth
- Genesis 22:10 (Title)
- Diptera Sonata (Basement) [Guitar]
- Crusade (Basic Boss Fight)
- Sodden Hollow (Caves) [Bass Guitar]
- The Forgotten (Secret Room)
- Abyss (The Depths) [Ambient Sounds]
- Matricide (Mom Fight)
- The Calm (Boss Defeated)
- Viscera (The Womb and Utero) [Drums]
- Ventricide (Womb Fight)
- Acceptance (Isaac Dies)
- Anima Vendit (Devil Room)
- Duress (Sheol) [Drums]
- Hericide (Satan Fight)
- Murmur of the Harvestman (Store)
- Empty Vessels (Angel Room)
- Everlasting Hymn (Cathedral) [No Change]
- Infanticide (Isaac Fight)
- Ambush (Boss Rush)
- Tome of Knowledge (Library)
- Periculum (The Cellar) [Guitar]
- Capiticus Calvaria (Catacombs) [Guitar]
- When Blood Dries (Necropolis) [Guitar]
- Sketches of Pain (The Chest) [Guitar]
- Ascension (??? (Boss) Fight)
- Devoid (Dark Room) [Ambient Sounds]
- The Fallen Angel (The Lamb Fight)
- Tribute (Credits)
- Genesis 13:37 (Arcade)
- Hush (Jesus Loves Uke)
- Descensum (BONUS)
- He's the Number One (BONUS)
The Binding of Isaac: Afterbirth
- ReGenesis (Updated Title Theme)
- Cerebrum Dispersio (Boss Fight - Alternate)
- Cicatrix (Scarred Womb) [Drums]
- Fundamentum (Burning Basement) [Guitar]
- Kave Diluvii (Flooded Caves) [Guitar]
- Nativitate (Blue Womb)
- Morituros (Hush Fight)
- Pulso Profundum (Dank Depths) [Drums]
- Chorus Mortis (Ultra Greed Fight)
The Binding of Isaac: Afterbirth+
The Binding of Isaac: Repentance
- Genesis Retake Light (Updated Title Theme)
- River Of Despair (Downpour)
- Juggernaut (Boss Fight - Alternate)
- Fissure (Mines)
- Super Secret Room
- Walls Of Denial (Mausoleum)
- Dread (The Calm Twisted)
- Carrion (Corpse)
- Morticide (Mother Fight)
- Caesarian (Utero)
- Armageddon (Boss Rush)
- StarGazer (Planetarium)
- Night Soil (Dross)
- Absentia (Ashpit)
- Morning Star (Gehenna)
- Bygone (Ultra Secret Room)
- Home
- Living In The Light (Dogma Fight)
- Revelations 13-1 (The Beast Fight)
- Genesis Retake Dark (Updated Title Theme - Tainted Character Selection)
Trivia
- The title screen theme of The Legend of Bum-Bo is a remix of the Binding of Isaac: Rebirth title theme with the same name.
- The Binding of Isaac: Lullabies was an additional album released by Ridiculon. While these specific tracks do not appear in the game, the music consists of lullaby variants of the official soundtrack.
- In an interview on Steam by Tyrone Rodriguez of Ridiculon producers Matthias Bossi and Jon Evans, Jon mentioned that every track made for Repentance is his favorite. However, he pointed out three OSTs in particular as what he's most proud of: "'Sodden Hollow' for its dripping groove," "'Sheol' for even being allowed to be so terrifying in a game OST," and "'Delirium' for its dank heaviness."
- The Boss Rush Theme Armageddon seems to be a remix of the songs "Cerebrum Dispersio" and "Crusade", the standard boss battle themes, with many unique elements mixed in.
- The Blue Womb's theme features a combat layer in the OST and the game files, however this is unused due to the floor having no enemies aside from the boss.
With the Red Key, it is possible to generate red rooms and the combat layer can be heard in them.- It can also be heard in DELETE THIS if the random track selected is this theme.
 The Binding of Isaac: Rebirth
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