Multi-segmented enemies will only take damage on one segment.
Using it while playing as Tainted Jacob will split Dark Esau. The Esaus are able to damage each other, and will likely die quickly unless they are separated somehow. Upon their deaths, Jacob will not be turned into the ghost form.
Lusty Blood: Each split enemy killed grants a damage boost.
Sigil of Baphomet: Split enemies will allow the shield to activate and stack up more easily.
Blood Puppy: If Blood Puppy is angry, splits it into two copies. One copy can level up and be tamed like normal, but the other cannot level up and dies instead of being tamed.
Book of Virtues: Spawns one red wisp on the middle ring on use. Before spawning wisps, Meat Cleaver divides all of Isaac's wisps into two wisps that have half health and deal half tear damage. Wisps will be destroyed if they try to divide a third time.
This item's pickup quote is a reference to the phrase "Slice and Dice", which means to cut or divide something into smaller parts.
PC E1CT EDJH (Treasure Room adjacent to spawn)
If used on Reap Creep, during their 3rd Phase, they cannot properly collide with the walls in order to perform their rock attack and will infinitely run against the wall.
If used against Mom's foot, both will count as their own instance, and the second foot will only be able to be killed once the first one is dead.