Locust of Death is an unlockable trinket added in The Binding of Isaac: Afterbirth † expansion.
- Upon entering a room with enemies, spawns a Black Locust that deals quadruple Isaac's damage, applied as two hits of double damage.
- The Locust will also spawn each time a wave starts in Boss Rush.
- The Locust will not spawn each time a wave in Greed Mode starts.
- If the player has exited the game, the Locust will spawn upon continuing from the main menu, allowing multiple locusts to spawn for the same room by repeatedly exiting.