Spawns a wisp that orbits Isaac, fires tears, blocks enemy shots, and deals contact damage to enemies, much like the ones spawned by Book of Virtues.
The jar initially spawns only one wisp, but spawns an additional wisp each time the jar is used, up to a maximum of 12 per use. The number of wisps that will be spawned by the jar is indicated by both a counter and the jar visually filling up with wisps.
The wisps spawned by the jar can be ones created by any Book of Virtues synergy, although basic wisps are much more common than special variants.
Isaac can have up to 3 rings of wisps; an inner, counter-clockwise spinning ring of 6 wisps, a center, clockwise-spinning ring of 8 wisps, and an outer, counter-clockwise spinning ring of 12 wisps, for a total of 26.
Bomb wisps with a gray tint are not dangerous; Doctor's Remote wisps only invoke their special effect when said item is used.
Wisps that look like gray cubes will invoke the effect of the D7 when they take damage.
Teleport!/Teleport 2.0 Wisps fade into and out of existence and have a chance to teleport non-boss enemies to a random spot in the current room when their tears hit them.
Switching the jar with other active items and then picking it up again does not reset the number of wisps that will be spawned on use.
Each use of Glowing Hourglass that does not move Isaac to a different room from the one he is currently in (such as by teleporting to the previous room then using it again) will duplicate the wisps. Any extra wisps created from duplication on a filled ring will appear at the entrance of each room Isaac enters and shoot in the direction Isaac is currently shooting.