Binding of Isaac: Rebirth Wiki
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(except in Rebirth)

Jar of Flies is an activated item added in The Binding of Isaac: Afterbirth.

Effects[ | ]

  • Killing an enemy adds a fly to the jar, up to a maximum of 20.
  • Upon use, releases Blue Flies equal to the number of flies in the jar.
  • This item belongs to the BeelzebubBeelzebub set. Collecting three items from this set will transform Isaac into a giant humanoid fly.

Notes[ | ]

  • Kills by blue flies and locusts (including flies from the jar) and certain familiars (including (except in Repentance)Collectible Incubus iconIncubus and the launch attack from (in Repentance) Character Tainted Lilith iconTainted Lilith's fetus) do not add flies to the jar.
Items that add to Jar of Flies
(in Afterbirth † and Repentance)Collectible 7 Seals icon7 Seals contact
(in Repentance)Collectible Abyss iconAbyss locusts
(in Afterbirth † and Repentance)Collectible Angry Fly iconAngry Fly
(in Repentance)Apollyon's Best FriendApollyon's Best Friend
Collectible Ball of Bandages iconBall of Bandages contact
Collectible BBF iconBBF
Collectible Big Fan iconBig Fan
(in Afterbirth † and Repentance)Collectible Bloodshot Eye iconBloodshot Eye contact
Blue Spiders
Collectible Bob's Brain iconBob's Brain
(in Afterbirth † and Repentance)Collectible Book of the Dead iconBook of the Dead bone orbitals
(in Afterbirth † and Repentance)Collectible Book of the Dead iconBook of the Dead bony contact
(in Repentance)Collectible Book of Virtues iconBook of Virtues / (in Repentance)Collectible Jar of Wisps iconJar of Wisps Wisp contact
Collectible Bumbo iconBumbo level 4
(in Repentance)Collectible Cain's Other Eye iconCain's Other Eye
Collectible Daddy Longlegs iconDaddy Longlegs
(in Afterbirth † and Repentance)Collectible Depression iconDepression
Collectible Distant Admiration iconDistant Admiration
Collectible Dry Baby iconDry Baby
Collectible Farting Baby iconFarting Baby
(in Afterbirth † and Repentance)Collectible Finger! iconFinger!
File:Collectible Forever Alone icon.pngForever Alone
(in Repentance)Found SoulFound Soul
Collectible Friend Zone iconFriend Zone
Friendly enemy contact
Friendly enemy lasers
(in Repentance)Collectible Fruity Plum iconFruity Plum
(in Repentance)Collectible Gello iconGello tears
(in Repentance)Collectible Ghost Bombs iconGhost Bombs ghosts
Collectible Guillotine iconGuillotine orbital
Collectible Guppy's Hairball iconGuppy's Hairball
Collectible Headless Baby iconHeadless Baby
Collectible Holy Water iconHoly Water
(in Repentance)Collectible Hungry Soul iconHungry Soul ghosts
(in Afterbirth † and Repentance)Collectible Hushy iconHushy
(in Repentance)Collectible Incubus iconIncubus
(in Repentance)Collectible The Intruder iconThe Intruder contact
Collectible Leech iconLeech
(in Repentance)Collectible Lemegeton iconLemegeton wisps
(in Repentance)Collectible Lil Abaddon iconLil Abaddon
Collectible Lil Brimstone iconLil Brimstone
(in Repentance)Lil ClotLil Clot
Collectible Lil Gurdy iconLil Gurdy
Collectible Lil Haunt iconLil Haunt
(in Afterbirth † and Repentance)Collectible Lil Spewer iconLil Spewer
Collectible Lost Fly iconLost Fly
(in Afterbirth † and Repentance)Collectible Mom's Razor iconMom's Razor
Collectible Mongo Baby iconMongo Baby copying one in this list
Collectible My Shadow iconMy Shadow
Collectible Obsessed Fan iconObsessed Fan
Collectible The Peeper iconThe Peeper
Collectible The Pinking Shears iconThe Pinking Shears body
(in Afterbirth † and Repentance)Collectible Pointy Rib iconPointy Rib
(in Repentance)Collectible Quints iconQuints babies that copy one in this list
Collectible Robo-Baby iconRobo-Baby
Collectible Robo-Baby 2.0 iconRobo-Baby 2.0
Collectible Sacrificial Dagger iconSacrificial Dagger
Collectible Samson's Chains iconSamson's Chains
(in Repentance)Collectible Scissors iconScissors
(in Repentance)Collectible The Scooper iconThe Scooper
(in Afterbirth † and Repentance)Collectible Shade iconShade
Collectible Smart Fly iconSmart Fly
Collectible Spider Mod iconSpider Mod burn and poison
(in Afterbirth † and Repentance)Collectible Sprinkler iconSprinkler
(in Repentance)Collectible Stitches iconStitches
Collectible Succubus iconSuccubus
(in Repentance)Collectible Sumptorium iconSumptorium clot tears
(in Repentance)Collectible Tinytoma iconTinytoma
(in Repentance)Collectible Vengeful Spirit iconVengeful Spirit contact
(in Repentance)Collectible Worm Friend iconWorm Friend
Items that don't add to Jar of Flies
Collectible Blue Baby's Only Friend icon???'s Only Friend
???'s Soul???'s Soul
Collectible Abel iconAbel
Collectible Ball of Bandages iconBall of Bandages tears
Collectible Best Bud iconBest Bud
(in Afterbirth † and Repentance)Collectible Big Chubby iconBig Chubby
(in Repentance)Collectible Bird Cage iconBird Cage
(in Afterbirth † and Repentance)Collectible Bloodshot Eye iconBloodshot Eye tears
Blue Flies
(in Repentance)Collectible Boiled Baby iconBoiled Baby
(in Afterbirth † and Repentance)Collectible Book of the Dead iconBook of the Dead bony shots
(in Repentance)Collectible Book of Virtues iconBook of Virtues / (in Repentance)Collectible Jar of Wisps iconJar of Wisps wisp tears
(in Repentance)Collectible Bot Fly iconBot Fly
Collectible Brother Bobby iconBrother Bobby
(in Afterbirth † and Repentance)Collectible Brown Nugget iconBrown Nugget
(in Afterbirth † and Repentance)Collectible Buddy in a Box iconBuddy in a Box
Collectible Bumbo iconBumbo level 3 tears
(except in Repentance)Collectible Cain's Other Eye iconCain's Other Eye
Charmed enemies
(in Repentance)Collectible Cube Baby iconCube Baby
Collectible Cube of Meat iconCube of Meat
Collectible Dead Bird iconDead Bird
Collectible Demon Baby iconDemon Baby
Eve's Bird FootEve's Bird Foot
Collectible Fate's Reward iconFate's Reward
(in Repentance)Collectible Freezer Baby iconFreezer Baby
Friendly enemy tears
(in Repentance)Collectible Gello iconGello whip
Collectible Gemini iconGemini
Collectible Ghost Baby iconGhost Baby
(in Repentance)Collectible Giant Cell iconGiant Cell
Collectible Guardian Angel iconGuardian Angel
Collectible Halo of Flies iconHalo of Flies
Collectible Harlequin Baby iconHarlequin Baby
(except in Repentance)Collectible Incubus iconIncubus
(in Repentance)Collectible The Intruder iconThe Intruder tears
Isaac's HeadIsaac's Head
Collectible Isaac's Heart iconIsaac's Heart
(in Afterbirth † and Repentance)Collectible Jaw Bone iconJaw Bone
(in Repentance)Collectible Knife Piece 1 iconKnife Piece 1 & (in Repentance)Collectible Knife Piece 2 iconKnife Piece 2
(in Repentance) Knockback damage (e.g. Collectible Butter Bean iconButter Bean)
(in Afterbirth † and Repentance)Collectible Leprosy iconLeprosy
(in Repentance)Collectible Lil Dumpy iconLil Dumpy
Collectible Lil Loki iconLil Loki
(in Afterbirth † and Repentance)Collectible Lil Monstro iconLil Monstro
(in Repentance)Collectible Lil Portal iconLil Portal
Collectible Little Chubby iconLittle Chubby
Collectible Little Gish iconLittle Gish
Collectible Little Steven iconLittle Steven
Collectible Mongo Baby iconMongo Baby's base tears or copying any familiar in this list
Collectible Papa Fly iconPapa Fly
(in Repentance)Collectible Psy Fly iconPsy Fly
(in Repentance)Collectible Purgatory iconPurgatory ghosts
(in Repentance)Collectible Quints iconQuints babies that copy any familiar in this list
Collectible Rainbow Baby iconRainbow Baby
(except in Repentance)Collectible Scissors iconScissors head
Collectible Seraphim iconSeraphim
Collectible Sister Maggy iconSister Maggy
(in Afterbirth † and Repentance)Collectible Slipped Rib iconSlipped Rib
Collectible Spider Mod iconSpider Mod shrink
(in Repentance)Collectible Spin to Win iconSpin to Win orbital
Collectible Sworn Protector iconSworn Protector
(in Repentance)Collectible Uranus iconUranus
(in Repentance)Collectible Vade Retro iconVade Retro ghosts
(in Repentance)Collectible Vengeful Spirit iconVengeful Spirit tears
  • Friendly enemies such as from the Collectible Friendly Ball iconFriendly Ball add flies to the jar, but not those spawned from other enemies.
  • Batteries do not affect the number of flies in the jar.
  • Enemies must be actually killed to add a fly; transformations (such as GaperGapers turning into PacerPacers) or knockouts (such as incapacitating a GlobinGlobin) do not count as kills.
  • Destroying a ShopkeeperShopkeeper will count as a kill.
  • With items that kill normally unkillable enemies, killing things like Stone GrimaceStone Grimaces will add flies to the jar.
  • Killing minions in the Hush fight does not add flies to the jar.

Synergies[ | ]

  • (in Repentance)Collectible Book of Virtues iconBook of Virtues: Using Jar of Flies summons wisps instead of Blue Flies; the wisps do not summon the flies when destroyed. Up to five flies at a time are consumed to summon one wisp, with stacking health and damage. The damage of the wisps decreases as they take damage. Enemies killed by wisps’ tears will not add flies to the jar.
  • (in Afterbirth † and Repentance)Fish TailFish Tail: Doubles the number of flies that spawn.
  • Collectible Hive Mind iconHive Mind: Flies are bigger and deal double damage.
  • (in Afterbirth † and Repentance)Collectible Schoolbag iconSchoolbag: Flies will be gained even while the Jar of Flies is in the inactive slot.

Interactions[ | ]

  • (in Repentance)Ace Cards: Enemies that are turned into pickups do not grant extra flies.
  • Collectible Car Battery iconCar Battery: No effect.
  • Collectible E. Coli iconE. Coli/Collectible Flush! iconFlush!: Enemies that are turned into poop do not grant extra flies.
  • (in Repentance)Collectible Uranus iconUranus: Enemies frozen by Uranus do not grant extra flies, even if shattered. Only enemies killed with bombs will add flies.
  • (in Afterbirth † and Repentance)Collectible Void iconVoid: Flies will not collect when absorbed by Void, making the interaction worthless.
  • Collectible Infestation 2 iconInfestation 2: Blue Spiders spawned by Infestation 2 grant extra flies, and Blue Flies spawned by Jar of Flies spawn Blue Spiders.

In-game Footage[ | ]

Trivia[ | ]

  • (except in Repentance) The item's pickup quote references the tagline of the Pokémon games. The quote said "Gotta catch 'em all?"
  • (in Afterbirth † and Repentance) With Booster Pack #2, this item's sprite fills up with more flies depending on how many flies it contains. This is based on this Steam Workshop mod by Stewartisme.
  • The item is likely a reference to the Alice In Chains album of the same name.

Bugs[ | ]

Bug Bug! (except in Repentance) The synergy of Collectible The Ludovico Technique iconThe Ludovico Technique with Collectible Brimstone iconBrimstone or Collectible Technology iconTechnology will not spawn flies.


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