Binding of Isaac: Rebirth Wiki

Infestation 2 is a passive item.


  • Spawns an allied Blue Spider from each enemy that is killed.
  • Added in RepentanceThis item belongs to the Spider Baby set. Collecting three items from this set will transform Isaac into a humanoid spider.


  • Enemies do not spawn Blue Spiders when killed by anything not controlled by Isaac (environmental hazards, charmed enemies, death upon clearing room, etc).
  • Enemies do not spawn Blue Spiders when killed by Blue Spiders.
  • Blue Flies can also spawn Blue Spiders.
  • Enemies killed by familiars will spawn Blue Spiders.
  • Destroying dead Shopkeeper Shopkeepers will spawn Blue Spiders.
  • Killing Pin Pin will give one Blue Spider for each of his segments.
  • Killing Chub Chub will spawn a lot of Blue Spiders.
  • Killing a Homunculus Homunculus or a Begotten Begotten will sometimes spawn a Blue Spider for each segment in the chains holding them to the wall.
  • Added in Afterbirth Dark Ball Dark Balls spawned by Added in Afterbirth Little Horn Little Horn can also drop Blue Spiders upon death.

In-game Footage[]



Vita F2YP 4L9V (Treasure Room adjacent to spawn)

PC KLFM X14Z (First floor Treasure Room)