Binding of Isaac: Rebirth Wiki

E. Coli is a passive item.


  • Enemies that touch Isaac are transformed into poop.
  • Added in Afterbirth This item belongs to the Oh Crap set. Collecting three items from this set will transform Isaac into a walking pile of poop.


  • Bosses cannot be turned into poop.
  • Poop created by this item is not grid-aligned, and flashes red when hit, similar to damaging monsters.
    • Added in Afterbirth † Poops produced by E. Coli crumble upon being attacked, identically to regular poops.
  • If you touch the middle section of a Grub with E. Coli, it will then split and become two heads (rather than destroying and spawning Maggots).
  • Mullibooms can be turned into poop without taking damage.
  • The poops vanish if the room is left and then returned to.
  • Added in Afterbirth / Removed in Repentance Poops created by E. Coli do not heal Isaac when he has the Oh Crap transformation.
  • Added in Repentance Poops created by E. Coli heal Isaac when he has the Oh Crap transformation.


  • Isaac's Heart Isaac's Heart: Isaac can turn enemies into poop without taking damage.
  • Holy Mantle Holy Mantle: On-contact effect still applies to enemies if Holy Mantle negates the contact damage taken.
  • Midas' Touch Midas' Touch: Enemies touched are turned into Golden Poop.
  • Unicorn Stump Unicorn Stump: Provides invincibility during which enemies can be touched.
  • Added in RepentanceLodestone Lodestone: If an enemy is hit by a lodestone tear perfectly simultaneously with it being turned into poop, the resulting poop will be tinted black and have a lodestone aura that sucks in enemies. This poop will return to normal after a few seconds.
  • Added in Repentance Tainted Forgotten Tainted Forgotten: The body will turn enemies into poop on contact.


  • Added in AfterbirthBrown Cap Brown Cap: The generated poops are affected and will explode when destroyed.
  • Added in Afterbirth †Hallowed Ground Hallowed Ground: The generated poops cannot be white.
  • Added in Afterbirth †Meconium Meconium: The generated poops cannot be black.
  • Added in AfterbirthSerpent's Kiss Serpent's Kiss / The Virus The Virus: The poops won't drop Black Hearts, negating the touch effect from these items on non-bosses.

In-game Footage[]


  • Escherichia coli is a type of intestinal bacteria present naturally in the human intestinal system. However, some harmful strains of E. Coli can cause food poisoning.
  • This item is used in High Brow (challenge #2) and Added in Repentance Scat Man (challenge #36).


PC DXGN OP4D (Treasure Room adjacent to spawn)

PS4 F1WD FBBN (Treasure Room adjacent to spawn)

Switch Greed mode only 8YRM FTHE (First Golden Treasure Room)