Creep is a liquid created by the attacks of certain monsters and bosses, or may be spawned by Isaac. Creep appears on the ground as a glowing or "shiny" puddle or line. There are a few different kinds of Creep which can affect enemies or Isaac. Having the ability to fly or the Callus trinket nullifies the effects of Creep.
Creep that will affect Isaac is shown flickering rapidly. When all enemies inside a room are defeated, the creep they produced will quickly disappear.
Red Creep is the most common type of Creep in the game. It can be created by various different enemies, champions, or Isaac himself. When spawned by enemies, it damages Isaac but not other enemies in the room. When spawned by Isaac, it damages walking enemies but not Isaac himself. It deals half a heart of damage to the player. Red Creep is representative of blood.
Green Creep is spawned by various enemies, Ultra Pride, Pestilence, green champion monsters, and Dangle and Brownie (when they are poisoned). When spawned by enemies, it is functionally equivalent to Red Creep, however some forms spawned by Isaac deal damage at a faster rate. It can be spawned by Isaac with Bob's Bladder, Toxic Shock, Mysterious Liquid, or 7 Seals' Pestilence familiar. (Notably, the Bob transformation actually generates red creep that is just colored green.) Green Creep is representative of acid, bile, or poison.
Yellow Creep is spawned only by Peep, Lemon Mishap, Lemon Party pills, and Free Lemonade. When spawned by Peep, it deals half a heart of damage to the player. When spawned by Isaac, it continuously damages walking enemies and seems to do more damage than any other type of creep that Isaac can create. Yellow Creep is representative of urine.
Black Creep is spawned by various enemies, Gish, the Something's Wrong... pill, Ball of Tar, and 7 Seals' Famine familiar. It slows the movement speed of affected enemies or the player. When spawned by enemies, it affects Isaac but not other enemies. When spawned by Isaac, it affects enemies but not Isaac, bypassing the status resistances of Bosses. Black Creep is likely representative of tar or oil, though in the case of the Something's Wrong... pill, it may be fecal matter with blood in it.
White Creep is functionally similar to Black Creep. White Creep is spawned by several bosses, including Widow, The Wretched, Triachnid, and The Frail, as well as Small Baby Long Legs. Isaac can spawn white creep with Juicy Sack, Large Zit, and Parasitoid. White Creep could be representative of a liquid form of Cobweb.
Blue Creep is spawned by Depression, or Holy Water. Aquarius also causes Isaac to leave a trail of blue creep wherever he walks. Blue creep created by Isaac damages enemies that walk over it. It is likely representative of water or tears.
Blue creep spawned by Holy Water petrifies enemies.
Blue creep spawned by Aquarius synergizes with tear effects.
Dark Blue Creep
Grey Creep is spawned only by The Cage. It damages Isaac like Red Creep but does not affect other enemies. It may be representative of some type of rotten fluid.
Orange Creep is spawned only by Mama Gurdy. It slows down Isaac like Black Creep, but does not affect other enemies. Unlike other types of Creep, Orange Creep does not flicker rapidly, even though it only affects Isaac. Due to its nature, it may be representative of vomit, pus, or some other bodily liquid.
Brown Creep is spawned by Tainted ??? and IBS's mechanic, or by bosses such as Brownie, Dangle, Turdlings, X-Lax pill, and Sisters Vis. Hostile Brown Creep and the Creep spawned by X-Lax will make Isaac slide, affecting his ability to stop moving or change directions while moving. This kind of Brown Creep does not flicker rapidly even though it only affects Isaac. Friendly Brown Creep spawned by Tainted ??? or IBS would give damage and tears boost if Isaac is standing on it, and will synergize with other kinds of poop spawned by those mechanic. It is representative of liquid poop, likely to be diarrhea.
Static Creep is spawned exclusively by Dogma's first phase. It appears underneath the boss as they move. It functions the same as Red Creep, harming Isaac for half a heart. Rather than being representative of a certain type of liquid, it represents visual static, most commonly seen on televisions with no signal.
Trail of Red Creep, spawned by Headless Baby.
Green Creep, spawned by Mysterious Liquid.
Yellow Creep, spawned by Lemon Mishap.
Trail of slowing Black Creep, spawned by Ball of Tar.
Trail of slowing White Creep, spawned by Juicy Sack.
Trail of Blue Creep, spawned by Aquarius.
Trail of Brown Creep, spawned by Brownie.
Static Creep, left behind by Dogma's first phase.
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