Chapters are sets of floors which contain similar enemies, themes, bosses, and difficulties. Earlier chapters are divided into three floors, while later chapters contain one floor. Each chapter has different environments which, while retaining the same feel, have slightly different themes, enemies, bosses, and difficulties. Each environment also has its own music.
Chapter 1[]
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 Basement
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 Cellar
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  Burning Basement
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Chapter 1.5 (Alternative)[]
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  Downpour
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 Dross
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Chapter 2[]
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 Caves
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 Catacombs
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 Flooded Caves
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Chapter 2.5 (Alternative)[]
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 Mines
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 Ashpit
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Chapter 3[]
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 Depths
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 Necropolis
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 Dank Depths
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Chapter 3.5 (Alternative)[]
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 Mausoleum
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 Gehenna
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Chapter 4[]
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 Womb
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 Utero
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 Scarred Womb
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Chapter 4.5 (Optional/Alternative)[]
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 ???
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 Corpse
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Chapter 5[]
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 Cathedral
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 Sheol
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Chapter 6 (Optional)[]
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 Chest
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 Dark Room
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Endgame (Optional)[]
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  The Void
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 Home
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Level Generation[]
For the technical details of how each level / floor is generated, see Level Generation.
Gallery[]
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A flowchart of how to unlock and reach each chapter
 Chapters
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| Chapter 1 | Chapter 2 | Chapter 3 | Chapter 4 | Optional | Chapter 5 | Chapter 6 | Endgame | |
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Basement |
 Caves |
 Depths |
 Womb |
 ??? |
 Sheol
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 Dark Room
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 The Void
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Basement |
Caves |
Depths |
Womb |
Sheol |
 The Shop |
 Ultra Greed | |
 The Binding of Isaac: Rebirth
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