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Dark Room[]

Boss The Lamb portrait.png

Chest[]

Golden Door[]

Mega Satan will always appear in the room inside the golden door found in The Chest and the Dark Room.

Boss Mega Satan portrait.png

Gemini • Steven • Blighted Ovum • Dingle • The Duke of Flies • Famine • Gurglings • The Haunt • Larry Jr. • Monstro • Pin • Widow • The Fallen • The Headless Horseman
Dangle • Little Horn • Rag Man • Turdlings
Baby Plum

Lil Blub • Wormwood • The Rainmaker • Min-Min • Clog • Colostomia • Turdlet

Fistula • Chub • C.H.A.D. • Carrion Queen • Dark One • Gurdy • Gurdy Jr. • The Hollow • The Husk • Mega Fatty • Mega Maw • Peep • Pestilence • Polycephalus • The Wretched • The Fallen • The Headless Horseman
The Forsaken • The Frail • The Stain
Big Horn • Rag Mega
Bumbino

Reap Creep • Tuff Twins • Hornfel • Great Gideon • Singe • The Shell • The Pile

The Adversary • The Bloat • The Cage • The Gate • Monstro II • Gish • Loki • Mask of Infamy • War • The Fallen • The Headless Horseman • Mom
Brownie
Sisters Vis
Reap Creep • The Pile

The Siren • The Heretic • The Visage • The Horny Boys • Mom • Mom's Heart

Blastocyst • The Bloat • Conquest • Daddy Long Legs • Triachnid • Death • Lokii • Mama Gurdy • Mr. Fred • Scolex • Teratoma • The Fallen • The Headless Horseman • Mom's Heart • It Lives
Sisters Vis • The Matriarch

Chimera • The Scourge • Rotgut • Mother

Satan • Isaac

??? • The Lamb • Mega Satan

Dogma • The Beast • Ultra Death • Ultra Famine • Ultra Pestilence • Ultra War

Ultra Greed
Ultra Greedier

The Binding of Isaac: Rebirth

Cards and Runes

Transformations

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