Challenges

Challenges are unique runs in the game. Isaac typically starts each challenge with one or more specific items which makes normal play more difficult. With few exceptions, Treasure Rooms are not available during challenges. Each challenge unlocks new content for the game, such as runes when completed. Players, with the advent of modding, have created their own Custom Challenges too.

General Strategy

Most challenges are designed to increase the difficulty of the game in a unique fashion. This doesn't mean all challenges are difficult, however; challenges like Computer Savvy are quite easy to beat. Some challenges do compensate for this by being especially punishing; Solar System is a good example of this. Each challenge has certain strategies that can be employed to make the run easier. However, there are a few strategies that can apply to all challenges.

  • Challenges can be played in co-op, allowing a second player to contribute to the run without being hindered in the same way Isaac is. This is especially useful in any challenge where Isaac is Blindfolded, as the second player will be allowed to fire tears normally.
    • Dlc r indicator.png If other players join the game in the first room for true co-op, they will not get to select a character and will be forced to play as the character the challenge has you play as, ex. for Blue Bomber, all players will be forced to play as Character ??? (Character) icon.png ??? no matter what. (This excludes untrue co-op because of co-op babies.)
  • Due to the lack of Treasure Rooms, Devil Rooms become much more valuable. Some challenges can be made much easier by making just one deal, but it is worth noting that other challenges reduce Isaac's health to the extent that Devil Deals become too risky.
    • Some rooms (including Crawl Spaces) may have Treasure Room items by default. Since these items are random, results can vary from run to run.
    • Because locked Treasure Rooms won't spawn, keys can be used far more liberally. Also, trinkets and items that generate more coins, along with upgraded Shops, can help Isaac acquire a greater range of powerful items.
  • Collectible The Bible icon.png The Bible instantly kills Mom and Mom's Heart / It Lives. All challenges require defeating Mom, and most require defeating Mom's Heart or It Lives, making this an extremely powerful item, especially in challenges where Mom or Mom's Heart / It Lives is the final boss.
    • Collectible Pandora's Box icon.png Pandora's Box will spawn The Bible if used in Womb II, Utero II, or Dlc a indicator.png Scarred Womb II, making it a reliable way to acquire The Bible.
  • Curse Rooms can spawn items, making them valuable.
  • Dlc na indicator.png In some challenges, Dice Rooms and the Collectible D4 icon.png D4 are very useful, allowing that challenge's starting items to be rerolled, thus removing most of the difficulty. NOTE: This changes depending on the challenge. Most challenge pages contain details on the results of rerolling.
  • Blindfolded challenges are arguably the most difficult challenges due to Isaac's inability to fire tears. There are several methods to work around this.
  • Dlc a indicator.png Collectible Chaos icon.png Chaos is a powerful item in challenges, as it can allow powerful items to be found more commonly, such as in the Shop or after defeating bosses.
  • Defeating a boss beyond the specified end of the challenge (e.g. defeating Mom's Heart when the goal is to defeat Mom) will still spawn the trophy.
    • Dlc a indicator.png In some longer challenges (e.g. XXXXXXXXL), using a Sacrifice Room until it teleports Isaac to the Dark Room is a viable strategy. Once in the Dark Room, the trophy can be spawned by defeating The Lamb.
      • Dlc a† indicator.png In a similar vein, all runs can be completed by killing Delirium, meaning that the player can beat a challenge through an alternative method such as defeating Hush then traveling to The Void; however, this is generally extremely difficult given the restrictions challenges place on the player.

Notes

  • Apart from the achievement related to beating the challenge, all achievements are locked. Also, no Donation Machines will appear in any challenge.
  • The challenge reward will not be unlocked unless the trophy is picked up. If a Devil Room or Angel Room appears allowing Isaac to go to Sheol or the Cathedral, the unlock will be forfeited.
  • Isaac will not start any challenge with Collectible The D6 icon.png The D6.

Challenges List

Name Conditions Goal Unlocked by Unlocks
1. Pitch Black
Character Isaac appearance.png
HUD heart red full.png HUD heart red full.png HUD heart red full.png
Curse of Darkness
Curse of the Unknown
Item Pool Treasure Room.png: yes
Item Pool Shop.png: no
Boss Mom.png
Default Achievement Rune of Hagalaz icon.png Rune of Hagalaz
2. High Brow
Character Isaac appearance.png
HUD heart red full.png HUD heart red full.png HUD heart red full.png
Starts with:
Collectible Number One icon.png Number One
Collectible E Coli icon.png E Coli
Collectible Butt Bombs icon.png Butt Bombs
Collectible Flush! icon.png Flush!
Trinket Petrified Poop icon.png Petrified Poop
Item Pool Treasure Room.png: no
Item Pool Shop.png: yes
Boss Mom.png
Default Achievement Rune of Jera icon.png Rune of Jera
3. Head Trauma
Character Isaac appearance.png
HUD heart red full.png HUD heart red full.png HUD heart red full.png
Starts with:
Collectible Tiny Planet icon.png Tiny Planet
Collectible Soy Milk icon.png Soy Milk
Collectible The Small Rock icon.png The Small Rock
Collectible Iron Bar icon.png Iron Bar
Item Pool Treasure Room.png: no
Item Pool Shop.png: yes
Boss Mom.png
Default Achievement Rune of Ehwaz icon.png Rune of Ehwaz
4. Darkness Falls
Character Eve appearance.png
HUD heart red full.png HUD heart red full.png
Starts with:
Collectible Pentagram icon.png Pentagram
Collectible Dead Bird icon.png Dead Bird
Collectible Whore of Babylon icon.png Whore of Babylon
Collectible Razor Blade icon.png Razor Blade
Collectible Sacrificial Dagger icon.png Sacrificial Dagger
Collectible Dark Matter icon.png Dark Matter
Item Pool Treasure Room.png: no
Item Pool Shop.png: yes
Boss Satan.png
Achievement Darkness Falls icon.png Dlc nr indicator.png Defeat Mom's Heart 11 times
Dlc r indicator.png Defeat Satan and ??? as Character Eve icon.png Eve
Achievement Rune of Dagaz icon.png Rune of Dagaz
5. The Tank
Character Magdalene appearance.png
HUD heart red full.png HUD heart red full.png HUD heart red full.png HUD heart red full.png
Starts with:
Collectible Infamy icon.png Infamy
Collectible Bucket of Lard icon.png Bucket of Lard
Collectible Thunder Thighs icon.png Thunder Thighs
Collectible Yum Heart icon.png Yum Heart
Item Pool Treasure Room.png: no
Item Pool Shop.png: yes
Boss Mom.png
Achievement The Tank icon.png Have 7 or more Red Heart Containers at one time Achievement Rune of Ansuz icon.png Rune of Ansuz
6. Solar System
Character Isaac appearance.png
HUD heart red full.png HUD heart red full.png HUD heart red full.png
Blindfolded.png Blindfolded
Starts with:
Collectible Distant Admiration icon.png Distant Admiration
Collectible Forever Alone icon.png Forever Alone
Collectible Halo of Flies icon.png Halo of Flies
Collectible Transcendence icon.png Transcendence
Item Pool Treasure Room.png: no
Item Pool Shop.png: yes
Boss Mom's Heart.png
Achievement Solar System icon.png Defeat Mom's Heart 3 times Achievement Rune of Perthro icon.png Rune of Perthro
7. Suicide King
Character Lazarus appearance.png
HUD heart red full.png HUD heart red full.png HUD heart red full.png
Starts with:
Collectible Ipecac icon.png Ipecac
Collectible Mr. Mega icon.png Mr. Mega
Collectible My Reflection icon.png My Reflection
1 random pill
Item Pool Treasure Room.png: no
Item Pool Shop.png: yes
Boss Isaac.png
Achievement Suicide King 2 icon.png Defeat Mom's Heart 11 times Achievement Suicide King icon.png Suicide King
8. Cat Got Your Tongue
Character Isaac appearance.png
HUD heart red full.png HUD heart red full.png HUD heart red full.png HUD heart black full.png HUD heart black full.png HUD heart black full.png HUD heart soul full.png HUD heart soul full.png HUD heart soul full.png
Blindfolded.png Blindfolded
Starts with:
Collectible Guppy's Hair Ball icon.png Guppy's Hair Ball
Collectible Guppy's Head icon.png Guppy's Head
Collectible Guppy's Tail icon.png Guppy's Tail
Item Pool Treasure Room.png: no
Item Pool Shop.png: yes
Boss Mom.png
Achievement Cat Got Your Tongue icon.png Become Guppy Achievement Rune of Algiz icon.png Rune of Algiz
9. Demo Man
Character Isaac appearance.png
HUD heart red full.png HUD heart red full.png HUD heart red full.png
Starts with:
Collectible Dr. Fetus icon.png Dr. Fetus
Collectible Remote Detonator icon.png Remote Detonator
Trinket Match Stick icon.png Match Stick
Item Pool Treasure Room.png: no
Item Pool Shop.png: yes
Boss Mom's Heart.png
Achievement Demo Man icon.png Defeat Mom's Heart 9 times Achievement Chaos Card icon.png Chaos Card
10. Cursed!
Character Magdalene appearance.png
HUD heart red full.png HUD heart red full.png HUD heart red full.png HUD heart red full.png
Starts with:
Collectible Blue Map icon.png Blue Map
Collectible The Compass icon.png The Compass
Collectible Raw Liver icon.png Raw Liver
Collectible Treasure Map icon.png Treasure Map
Collectible Yum Heart icon.png Yum Heart
Trinket Child's Heart icon.png Child's Heart
Curse of the Cursed
Item Pool Treasure Room.png: yes
Item Pool Shop.png: yes
Boss Mom.png
Achievement Cursed! icon.png Have 7 or more Red Heart Containers at one time Achievement Credit Card icon.png Credit Card
11. Glass Cannon
Character Judas appearance.png
HUD heart red full.png
Starts with:
Collectible Epic Fetus icon.png Epic Fetus
Collectible Loki's Horns icon.png Loki's Horns
Item Pool Treasure Room.png: no
Item Pool Shop.png: yes
Boss Satan.png
Achievement Glass Cannon icon.png Beat The Family Man (challenge #19) and defeat Lokii Achievement Rules Card icon.png Rules Card
12. When Life Gives You Lemons
Character Isaac appearance.png
HUD heart red full.png HUD heart red full.png HUD heart red full.png
Starts with:
Collectible 9 Volt icon.png 9 Volt
Collectible Habit icon.png Habit
Collectible Lemon Mishap icon.png Lemon Mishap
1 Lemon Party pill
Item Pool Treasure Room.png: no
Item Pool Shop.png: yes
Boss Mom.png
Default Achievement Card Against Humanity icon.png Card Against Humanity
13. Beans!
Character Isaac appearance.png
HUD heart red full.png HUD heart red full.png HUD heart red full.png
Blindfolded.png Blindfolded
Starts with:
Collectible 9 Volt icon.png 9 Volt
Collectible The Bean icon.png The Bean
Collectible The Black Bean icon.png The Black Bean
Collectible Butt Bombs icon.png Butt Bombs
Collectible Pyro icon.png Pyro
Item Pool Treasure Room.png: no
Item Pool Shop.png: yes
Boss Mom.png
Default Achievement Burnt Penny icon.png Burnt Penny
14. It's in the Cards
Character Isaac appearance.png
HUD heart red full.png HUD heart red full.png HUD heart red full.png
Starts with:
Collectible 9 Volt icon.png 9 Volt
Collectible The Battery icon.png The Battery
Collectible Deck of Cards icon.png Deck of Cards
Collectible Starter Deck icon.png Starter Deck
2 random tarot cards
Item Pool Treasure Room.png: no
Item Pool Shop.png: yes
Boss Mom.png
Default Achievement SMB Super Fan icon.png SMB Super Fan
15. Slow Roll
Character Isaac appearance.png
HUD heart red full.png HUD heart red full.png HUD heart red full.png
Starts with:
Collectible Cupid's Arrow icon.png Cupid's Arrow
Collectible My Reflection icon.png My Reflection
Collectible Polyphemus icon.png Polyphemus
Item Pool Treasure Room.png: no
Item Pool Shop.png: yes
Boss Mom.png
Default Achievement Swallowed Penny icon.png Swallowed Penny
16. Computer Savvy
Character Isaac appearance.png
HUD heart red full.png HUD heart red full.png HUD heart red full.png
Starts with:
Collectible Spoon Bender icon.png Spoon Bender
Collectible Technology icon.png Technology
Collectible Technology 2 icon.png Technology 2
Item Pool Treasure Room.png: no
Item Pool Shop.png: yes
Boss Mom.png
Default Achievement Robo-Baby 2.0 icon.png Robo-Baby 2.0
17. Waka Waka
Character Isaac appearance.png
HUD heart red full.png HUD heart red full.png HUD heart red full.png
Starts with:
Collectible Anti-Gravity icon.png Anti-Gravity
Collectible Strange Attractor icon.png Strange Attractor
Item Pool Treasure Room.png: no
Item Pool Shop.png: yes
Boss Mom.png
Default Achievement Death's Touch icon.png Death's Touch
18. The Host
Character Isaac appearance.png
HUD heart red full.png HUD heart red full.png HUD heart red full.png
Starts with:
Collectible The Mulligan icon.png The Mulligan
Collectible Spiderbaby icon.png Spiderbaby
Trinket Tick icon.png Tick
Item Pool Treasure Room.png: no
Item Pool Shop.png: yes
Boss Mom.png
Default Achievement Technology .5 icon.png Technology .5
19. The Family Man
Character Isaac appearance.png
HUD heart red full.png HUD heart red full.png HUD heart red full.png
Blindfolded.png Blindfolded
Starts with:
Collectible BFFS! icon.png BFFS!
Collectible Brother Bobby icon.png Brother Bobby
Collectible Dad's Key icon.png Dad's Key
Collectible Rotten Baby icon.png Rotten Baby
Collectible Sister Maggy icon.png Sister Maggy
Item Pool Treasure Room.png: no
Item Pool Shop.png: yes
Boss Isaac.png
Achievement The Family Man icon.png Pick up both Key Pieces from the Angels in one run Achievement Epic Fetus icon.png Epic Fetus
20. Purist
Character Isaac appearance.png
HUD heart red full.png HUD heart red full.png HUD heart red full.png
Item Pool Treasure Room.png: no
Item Pool Shop.png: yes
Boss Mom's Heart.png
Achievement Purist icon.png Defeat Mom Achievement Rune of Berkano icon.png Rune of Berkano
Dlc a indicator.png 21. XXXXXXXXL
Character Isaac appearance.png
HUD heart red full.png HUD heart red full.png HUD heart red full.png
Item Pool Treasure Room.png: yes
Item Pool Shop.png: yes
Every floor is the size of an XL Womb floor, but does not combine the floors of each chapter.
Boss Mom's Heart.png
Achievement XXXXXXXXL icon.png Defeat Mom Achievement Gold Heart icon.png Gold Heart
Dlc a indicator.png 22. SPEED!
Character Isaac appearance.png
HUD heart red full.png HUD heart red full.png HUD heart red full.png
Item Pool Treasure Room.png: yes
Item Pool Shop.png: yes
Everything is sped up like the Broken Watch effect.
Boss Mom's Heart.png
Achievement SPEED! icon.png Defeat Mom Achievement Get out of Jail Free Card icon.png Get out of Jail Free Card
Dlc a indicator.png 23. Blue Bomber
Character ??? appearance.png
HUD heart soul full.png HUD heart soul full.png HUD heart soul full.png
Blindfolded.png Blindfolded
Starts with:
Collectible Brother Bobby icon.png Brother Bobby
Collectible Kamikaze! icon.png Kamikaze!
Collectible Pyromaniac icon.png Pyromaniac
Collectible Mr. Mega icon.png Mr. Mega
Item Pool Treasure Room.png: no
Item Pool Shop.png: yes
Boss Satan.png
Achievement Blue Bomber icon.png Destroy 10 Tinted Rocks and defeat Mom's Heart 11 times Achievement Gold Bomb icon.png Gold Bomb
Dlc a indicator.png 24. PAY TO PLAY
Character Isaac appearance.png
HUD heart red full.png HUD heart red full.png HUD heart red full.png
Starts with:
Collectible Money = Power icon.png Money = Power
Collectible Sack of Pennies icon.png Sack of Pennies
50 coins
Item Pool Treasure Room.png: no
Item Pool Shop.png: yes
Every door costs 1 cent to enter. Isaac drops money when hit. Isaac dies if he run out of money.
Boss Isaac.png
Achievement PAY TO PLAY icon.png Defeat Isaac as Character Cain icon.png Cain Achievement 2 new pills icon.png 2 new pills
Dlc a indicator.png 25. Have a Heart
Character Isaac appearance.png
HUD heart red full.png HUD heart red empty.png HUD heart red empty.png HUD heart red empty.png HUD heart red empty.png HUD heart red empty.png
HUD heart red empty.png HUD heart red empty.png HUD heart red empty.png HUD heart red empty.png HUD heart red empty.png HUD heart red empty.png
Starts with:
Collectible Charm of the Vampire icon.png Charm of the Vampire
Item Pool Treasure Room.png: yes
Item Pool Shop.png: yes
Pennies drop instead of Red Hearts. Many items that restore Red Hearts have no effect.
Boss Mom's Heart.png
Achievement Have a Heart icon.png Defeat Mom Achievement 2 new pills icon.png 2 new pills
Dlc a indicator.png 26. I RULE!
Character Isaac appearance.png
HUD heart red full.png HUD heart red full.png HUD heart red full.png
Starts with:
Collectible The Boomerang icon.png The Boomerang
Collectible Key Piece 1 icon.png Key Piece 1
Collectible Key Piece 2 icon.png Key Piece 2
Collectible The Ladder icon.png The Ladder
Collectible Mom's Knife icon.png Mom's Knife
Collectible Trinity Shield icon.png Trinity Shield
Item Pool Treasure Room.png: no
Item Pool Shop.png: yes
Boss Mega Satan.png
Achievement I RULE! icon.png Dlc nr indicator.png Defeat ??? or The Lamb, and defeat Satan as Character Isaac icon.png Isaac
Dlc r indicator.png Defeat Mega Satan
Achievement Poker Chip icon.png Poker Chip
Dlc a indicator.png 27. BRAINS!
Character ??? appearance.png
HUD heart soul full.png HUD heart soul full.png HUD heart soul full.png
Blindfolded.png Blindfolded
Starts with:
3 Collectible Bob's Brain icon.png Bob's Brain
Collectible Thunder Thighs icon.png Thunder Thighs
Item Pool Treasure Room.png: no
Item Pool Shop.png: yes
Boss Blue Baby.png
Achievement BRAINS! icon.png Defeat Isaac 5 times Achievement Stud Finder icon.png Stud Finder
Dlc a indicator.png 28. PRIDE DAY!
Character Isaac appearance.png
HUD heart red full.png HUD heart red full.png HUD heart red full.png
Starts with:
Collectible 3 Dollar Bill icon.png 3 Dollar Bill
Collectible Rainbow Baby icon.png Rainbow Baby
Dlc a indicator.png Trinket Rainbow Worm icon.png Rainbow Worm
Item Pool Treasure Room.png: no
Item Pool Shop.png: yes
Cannot drop or replace Rainbow Worm.
Boss Mom's Heart.png
Achievement PRIDE DAY! icon.png Defeat Mom Achievement D8 icon.png D8
Dlc a indicator.png 29. Onan's Streak
Character Judas appearance.png
HUD heart red full.png
Starts with:
Collectible The Book of Belial icon.png The Book of Belial
Collectible Chocolate Milk icon.png Chocolate Milk
Item Pool Treasure Room.png: yes
Item Pool Shop.png: yes
Missing a shot deals damage to Judas.
Boss Isaac.png
Achievement Onan's Streak icon.png Defeat Mom Achievement Kidney Stone icon.png Kidney Stone
Dlc a indicator.png 30. The Guardian
Character Isaac appearance.png
HUD heart red full.png HUD heart red full.png HUD heart red full.png
Blindfolded.png Blindfolded
Starts with:
Collectible Holy Grail icon.png Holy Grail
Collectible Isaac's Heart icon.png Isaac's Heart
Collectible Punching Bag icon.png Punching Bag
Collectible Spear of Destiny icon.png Spear of Destiny
Item Pool Treasure Room.png: no
Item Pool Shop.png: yes
Isaac's Heart will follow Collectible Punching Bag icon.png Punching Bag instead of Isaac.
Boss Mom's Heart.png
Achievement The Guardian icon.png Defeat Mom Achievement Blank Rune icon.png Blank Rune
Dlc a† indicator.png 31. Backasswards
Character Isaac appearance.png
HUD heart red full.png HUD heart red full.png HUD heart red full.png
Starts with:
8-10 random items
Item Pool Treasure Room.png: no
Item Pool Shop.png: yes
All chapters progress in reverse order, losing 1 item and 1 Red Heart container each floor ascended.
Stage Basement icon.png Basement/ Stage Cellar icon.png Cellar/ Stage Burning Basement icon.png Burning Basement I Achievement Backasswards icon.png Dlc nr indicator.png Defeat ??? or The Lamb
Dlc r indicator.png Defeat Mega Satan
Achievement Laz Bleeds More! icon.png Laz Bleeds More!
Dlc a† indicator.png 32. Aprils Fool
Character Isaac appearance.png
HUD heart red full.png HUD heart red full.png HUD heart red full.png
Item Pool Treasure Room.png: yes
Item Pool Shop.png: yes
The minimap displays the floor's layout accurately, but displays Isaac's position inaccurately. Pedestal items and pickups are not what they appear to be. Active items, pills, cards, and rune effects are randomized upon each use, making them very unreliable or deadly (for example Suicide King). Items with no charge time (i.e. How To Jump, IV Bag, etc) can be used repeatedly for random effects. Stoneys have a chance of being replaced by Cross Stonies. Every boss is The Bloat. Isaac's health is not what it appears to be. Key and bomb numbers are accurate.
Boss Mom's Heart.png
Achievement Aprils fool icon.png Defeat Mom Achievement Maggy Now Holds a Pill! icon.png Maggy Now Holds a Pill!
Dlc a† indicator.png 33. Pokey Mans
Character Isaac appearance.png
HUD heart red full.png HUD heart red full.png HUD heart red full.png
Starts with:
Collectible Friendly Ball icon.png Friendly Ball
Collectible Mom's Eyeshadow icon.png Mom's Eyeshadow
Item Pool Treasure Room.png: no
Item Pool Shop.png: yes
Each use of Friendly Ball causes a random monster to be spawned. Friendly Ball instantly charges once the spawned monster is killed.
Boss Isaac.png
Achievement Pokey Mans icon.png Defeat Mom's Heart 11 times Achievement Charged Key icon.png Charged Key
Dlc a† indicator.png 34. Ultra Hard
Character Isaac appearance.png
HUD heart red full.png HUD heart red full.png HUD heart red full.png
Starts with:
Collectible Key Piece 1 icon.png Key Piece 1
Collectible Key Piece 2 icon.png Key Piece 2
Curse of the Labyrinth
Curse of the Blind
Curse of the Lost
Curse of the Maze
Item Pool Treasure Room.png: yes
Item Pool Shop.png: yes
All enemies spawn as champions if possible. Boss fights will be double trouble if possible. No heart pickups drop.
Boss Mega Satan.png
Achievement Ultra Hard icon.png Dlc nr indicator.png Defeat ??? or The Lamb. Possession of Collectible The Polaroid icon.png The Polaroid/ Collectible The Negative icon.png The Negative is required, barring getting to Stage Dark Room icon.png Dark Room through a Sacrifice Room.
Dlc r indicator.png Defeat Mega Satan
Achievement Samson Feels Healthy! icon.png Samson Feels Healthy!
Dlc a† indicator.png 35. Pong
Character Isaac appearance.png
HUD heart red full.png HUD heart red full.png HUD heart red full.png
Starts with:
Collectible Cupid's Arrow icon.png Cupid's Arrow
Collectible Rubber Cement icon.png Rubber Cement
Item Pool Treasure Room.png: no
Item Pool Shop.png: yes
Damage fixed at 100, tear delay fixed at 150.
Boss Blue Baby.png
Achievement PONG icon.png Defeat Isaac 5 times Achievement Greed's Gullet icon.png Greed's Gullet
Dlc r indicator.png 36. Scat Man
Character Isaac appearance.png
HUD heart red full.png HUD heart red full.png HUD heart red full.png HUD heart red full.png
Blindfolded.png Blindfolded
Starts with:
Collectible The Poop icon.png The Poop
Collectible 9 Volt icon.png 9 Volt
Collectible Skatole icon.png Skatole
Collectible E Coli icon.png E Coli
3 Collectible Butt Bombs icon.png Butt Bombs
Collectible BFFS! icon.png BFFS!
Collectible Thunder Thighs icon.png Thunder Thighs
Dlc r indicator.png Collectible Dirty Mind icon.png Dirty Mind
Trinket Mysterious Candy icon.png Mysterious Candy
Item Pool Treasure Room.png: no
Item Pool Shop.png: yes
Boss Mom.png
Default Achievement Dirty Mind icon.png Dirty Mind
Dlc r indicator.png 37. Bloody Mary
Character Bethany appearance.png
HUD heart red full.png HUD heart red full.png HUD heart red full.png HUD heart red full.png
Starts with:
Collectible The Book of Belial icon.png The Book of Belial
Collectible Blood Bag icon.png Blood Bag
Collectible Anemic icon.png Anemic
Dlc r indicator.png Collectible Blood Oath icon.png Blood Oath
Trinket Child's Heart icon.png Child's Heart

4 soul charges
Item Pool Treasure Room.png: no
Item Pool Shop.png: yes
Boss Satan.png
Achievement Bloody Mary icon.png Defeat Mom's Heart/It Lives on hard mode as Character Lazarus icon.png Lazarus, without dying, defeat Mom's Heart 11 times and unlock Collectible Blood Bag icon.png Blood Bag Achievement Sigil of Baphomet icon.png Sigil of Baphomet
Dlc r indicator.png 38. Baptism by Fire
Character Bethany appearance.png
HUD heart red full.png HUD heart red full.png HUD heart red full.png
Blindfolded.png Blindfolded
Starts with:
Dlc r indicator.png Collectible Urn of Souls icon.png Urn of Souls
Dlc a† indicator.png Collectible Schoolbag icon.png Schoolbag
Collectible Guppy's Paw icon.png Guppy's Paw
Trinket Maggy's Faith icon.png Maggy's Faith
Item Pool Treasure Room.png: no
Item Pool Shop.png: yes
Boss Isaac.png
Achievement Baptism by Fire icon.png Defeat Satan as Bethany, defeat Mom's Heart 11 times and unlock Trinket Maggy's Faith icon.png Maggy's Faith Achievement Purgatory icon.png Purgatory
Dlc r indicator.png 39. Isaac's Awakening
Character Isaac appearance.png
HUD heart red full.png HUD heart red full.png HUD heart red full.png
Starts with:
Dlc r indicator.png Collectible Spirit Sword icon.png Spirit Sword
Collectible Trinity Shield icon.png Trinity Shield
Dlc r indicator.png Collectible Mom's Bracelet icon.png Mom's Bracelet
Item Pool Treasure Room.png: no
Item Pool Shop.png: yes
Boss Mother.png
Achievement Isaac's Awakening icon.png Defeat Mother Achievement Spirit Sword icon.png Spirit Sword
Dlc r indicator.png 40. Seeing Double
Character Jacob appearance.png
HUD heart red full.png HUD heart red full.png HUD heart red full.png HUD heart red full.png HUD heart soul full.png
Starts with:
Jacob: Collectible 20 20 icon.png 20/20
Esau: Collectible 20 20 icon.png 20/20
Collectible X-Ray Vision icon.png X-Ray Vision
Item Pool Treasure Room.png: no
Item Pool Shop.png: yes
Boss Mom's Heart.png
Achievement Seeing Double icon.png Defeat Mother Achievement Broken Glasses icon.png Broken Glasses
Dlc r indicator.png 41. Pica Run
Character Isaac appearance.png
HUD heart red full.png HUD heart red full.png HUD heart red full.png
Starts with:
Dlc a indicator.png Collectible Mom's Box icon.png Mom's Box
Dlc a† indicator.png Collectible Marbles icon.png Marbles
Collectible Mom's Purse icon.png Mom's Purse
Item Pool Treasure Room.png: yes
Item Pool Shop.png: yes
Boss Isaac.png
Achievement Pica Run icon.png Defeat Mom's Heart 11 times and Unlock Marbles Achievement Ice Cube icon.png Ice Cube
Dlc r indicator.png 42. Hot Potato
Character Tainted Forgotten appearance.png
HUD heart soul full.png HUD heart soul full.png HUD heart soul full.png
Blindfolded.png Blindfolded
Item Pool Treasure Room.png: no
Item Pool Shop.png: yes
Boss Satan.png
Achievement The Fettered icon.png Use the Red Key or Cracked Key in Home as Character The Forgotten icon.png The Forgotten Achievement The Chariot icon.png The Chariot
Dlc r indicator.png 43. Cantripped!
Character Tainted Cain appearance.png
HUD heart red full.png HUD heart red full.png
Item Pool Treasure Room.png: no
Item Pool Shop.png: yes
Boss Mom.png
Achievement The Hoarder icon.png Use the Red Key or Cracked Key in Home as Character Cain icon.png Cain Achievement Justice icon.png Justice
Dlc r indicator.png 44. Red Redemption
Character Tainted Jacob appearance.png
HUD heart red full.png HUD heart red full.png HUD heart red full.png
Starts with:
Collectible Dad's Key icon.png Dad's Key
Dlc r indicator.png Collectible Red Key icon.png Red Key
Item Pool Treasure Room.png: yes
Item Pool Shop.png: yes
Boss Mother.png
Achievement The Deserter icon.png Use the Red Key or Cracked Key in Home as Character Jacob and Esau icon.png Jacob and Esau Achievement The Hermit icon.png The Hermit
Dlc r indicator.png 45. DELETE THIS
Character Isaac appearance.png
HUD heart red full.png HUD heart red full.png HUD heart red full.png
Starts with:
Dlc r indicator.png Collectible TMTRAINER icon.png TMTRAINER
Item Pool Treasure Room.png: yes
Item Pool Shop.png: yes
Boss Blue Baby.png
Achievement DELETE THIS icon.png Use the Red Key or Cracked Key in Home as Character Eden icon.png Eden Achievement Temperance icon.png Temperance

MainPageChallenges.png Challenges MainPageChallenges.png

Rebirth
1. Pitch Black 2. High Brow 3. Head Trauma 4. Darkness Falls 5. The Tank 6. Solar System 7. Suicide King
8. Cat Got Your Tongue 9. Demo Man 10. Cursed! 11. Glass Cannon 12. When Life Gives You Lemons 13. Beans! 14. It's in the Cards
15. Slow Roll 16. Computer Savvy 17. Waka Waka 18. The Host 19. The Family Man 20. Purist
Afterbirth
21. XXXXXXXXL 22. SPEED! 23. Blue Bomber 24. PAY TO PLAY 25. Have a Heart 26. I RULE! 27. BRAINS!
28. PRIDE DAY! 29. Onan's Streak 30. The Guardian
Afterbirth †
31. Backasswards 32. Aprils Fool 33. Pokey Mans 34. Ultra Hard 35. Pong
Repentance
36. Scat Man 37. Bloody Mary 38. Baptism by Fire 39. Isaac's Awakening 40. Seeing Double 41. Pica Run 42. Hot Potato
43. Cantripped! 44. Red Redemption 45. DELETE THIS
SmallIsaac.png The Binding of Isaac: Rebirth SmallIsaac.png
MainPageAchievements.png Achievements MainPageAttributes.png Attributes MainPageBosses.png Bosses TarotCard.png Cards and Runes MainPageChallenges.png Challenges MainPageChapters.png Chapters
Character Isaac appearance.png Characters MainPageBabies.png Co-op MainPageItems Small.png Items Collectible Isaac's Tears icon.png Item pools MainPageMonsters.png Monsters MainPageObjects.png Objects
Red Heart.png Pickups BlueBlue.png Pills MainPageRooms.png Rooms MainPageSeeds2.png Seeds Transformation Guppy appearance.png Transformations Trinket The Left Hand icon.png Trinkets
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