Binding of Isaac: Rebirth Wiki
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Challenge Rooms are special rooms that allow Isaac to fight enemies for extra rewards. They will only spawn on a floor if, upon entering the floor, Isaac's total health is greater than or equal to his number of heart containers. There are two kinds of challenge rooms: Normal Challenge Rooms, which appear on the first floor of a chapter or on Curse of the Labyrinth.png Curse of the Labyrinth floors, and Boss Challenge Rooms, which appear on the second floor of a chapter.

Normal Challenge Room[]

Challenge Room.png

Normal Challenge Rooms can only be entered if Isaac's total health (Red, Black, and Soul Hearts) is greater than or equal to his number of heart containers. Picking up its contents will cause three waves of enemies to spawn. While in chapter 4 (Womb Womb/Utero Utero/Scarred Womb Scarred Womb), 3 waves of bosses will be spawned, typically from Chapters 1 or 2.

Added in Afterbirth † Each wave cleared will charge Isaac's active item by 1.

Removed in Repentance Waves[]

The enemies that spawn depend on the current chapter, and each chapter has two different possible sets of enemies:

Floor Wave 1 Wave 2 Wave 3
Basement Basement 3 Hopper Hoppers 3 Horf Horfs 2 Clotty Clotties
5 Attack Fly Attack Flies 5 Pooter Pooters 4 Gaper Gapers
Cellar Cellar 3 Fatty Fatties 3 Round Worm Round Worms 5 Dip Dips
3 Gaper Gapers 3 Horf Horfs 3 Fatty Fatties
Caves Caves 5 Charger Chargers 4 Globin Globins 4 Maw Maws
5 Spitty Spitties 3 Hive Hives 3 Host Hosts
Catacombs Catacombs 2 Bony Bonies 2 Tumor Tumors 3 Wall Creep Wall Creeps
4 Round Worm Round Worms 3 Night Crawler Night Crawlers 1 Grub Grub
Depths Depths 3 Vis Vises 4 Leaper Leapers 4 Globin Globins
4 Baby Babies 5 Hopper Hoppers 4 Knight Knights
Necropolis Necropolis 4 Knight Knights 3 Mom's Hand Mom's Hands 3 Fat Sack Fat Sacks
4 Bony Bonies 2 Squirt Squirts 3 Nulls Nulls
Womb Womb
Utero Utero
3 Larry Jr. Larry Jr. Chub Chub and 3 Charger Chargers 2 Monstro Monstros
Fistula Fistula Loki Loki Chub Chub
Cathedral Cathedral
Sheol Sheol
3 Larry Jr. Larry Jr. Chub Chub and 3 Charger Chargers 2 Monstro Monstros
Fistula Fistula Loki Loki Chub Chub

Added in Repentance Waves (Sorted By Difficulty; Basement, Cellar, and Burning Basement Unused)[]

Floor Easy Normal Hard Hardest
Basement Basement 3 Hopper Hoppers / 5 Attack Fly Attack Flies / 4 Dip Dips 3 Horf Horfs / 5 Pooter Pooters / 2 3-Segment Army Fly Army Flies 2 Clotty Clotties / 4 Frowning Gaper Frowning Gapers / 1 Squirt Squirt and 2 Dip Dips 3 Level 2 Fly Level 2 Flies and 2 Full Fly Full Flies / 3 Sucker Suckers / 4 Cyclopia Cyclopias / 3 Ministro Ministros
Cellar Cellar 3 Level 2 Spider Level 2 Spiders / 5 Spider Spiders / 3 Sack Sacks 4 Trite Trites / 3 Small Crazy Long Legs Small Crazy Long Legs / 3 Wall Creep Wall Creeps 2 Nest Nests / 2 Crazy Long Legs Crazy Long Legs / 3 Small Baby Long Legs Small Baby Long Legs 2 Baby Long Legs Baby Long Legs / 2 Big Spider Big Spiders and 2 Spider Spiders / 2 Blind Creep Blind Creeps / 2 Migraine Migraines
Burning Basement Burning Basement 2 Flaming Hopper Flaming Hoppers 5 Dart Fly Dart Flies / 2 Pairs of 5 Swarm Swarms / 2 Pairs of 4 Swarm Spider Swarm Spiders 3 Flaming Fatty Flaming Fatties / 4 Sucker Suckers / 2 Grilled Clotty Grilled Clotties 4 Flaming Hopper Flaming Hoppers / 4 Crispy Crispies
Downpour Downpour 2 Blurb Blurbs / 5 Small Leech Small Leeches / 4 Strider Striders 4 Frowning Gaper Frowning Gapers and 1 Wraith Wraith / 2 Prey Preys / 3 Sub Horf Sub Horfs 5 Willo Willos and 2 Strider Striders / 2 Bubbles Bubbles and 2 Strider Striders / 3 Bloaty Bloaties 4 Sub Horf Sub Horfs and 2 Blurb Blurbs / 2 Mullighoul Mullighouls
Dross Dross 5 Drip Drips / 5 Small Leech Small Leeches / 2 Dump Head Dump Heads 3 Cloggy Cloggies / 2 Gas Dwarf Gas Dwarves and 4 Gas Clouds / 4 Big Corn Big Corns 4 Blurb Blurbs / 2 Bubbles Bubbles and 2 Sub Horf Sub Horfs / 3 Dump Dumps 2 Cloggy Cloggies and 1 Splurt Splurt / 2 Gas Dwarf Gas Dwarves and 2 Poot Mine Poot Mines / 4 Boom Fly Boom Flies and 4 Gas Clouds
Caves Caves 5 Conjoined Spitty Conjoined Spitties / 4 Boil Boils / 6 Maggot Maggots 3 Hive Hives / 3 Globin Globins / 4 Charger Chargers 3 Host Hosts / 3 Maw Maw / 2 Conjoined Fatty Conjoined Fatties 4 Pale Fatty Pale Fatties/ 2 Tumor Tumors / 4 Red Boom Fly Red Boom Flies
Catacombs Catacombs 5 Round Worm Round Worms / 4 Wall Creep Wall Creeps / 4 Splasher Splashers 3 Night Crawler Night Crawlers / 2 2-Segment Buttlicker Buttlickers and 1 3-Segment Buttlicker Buttlicker / 3 Boom Fly Boom Flies 1 3-Segment Grub Grub / 3 One Tooth One Tooths / 3 Psychic Horf Psychic Horfs 2 Mobile Host Mobile Hosts / 4 Chubber Chubbers / 4 Gurgle Gurgles
Flooded Caves Flooded Caves 4 Conjoined Spitty Conjoined Spitties / 3 Drowned Boom Fly Drowned Boom Flies 2 Drowned Hive Drowned Hives / 3 Globin Globins / 3 Night Crawler Night Crawler 4 Maw Maws / 2 Squirt Squirts / 2 Drowned Boom Fly Drowned Boom Flies and 2 Dukie Dukies 4 Blubber Blubbers and 2 Conjoined Fatty Conjoined Fatties / 5 Drowned Charger Drowned Chargers / 3 Tube Worm Tube Worms
Mines Mines 4 Soy Creep Soy Creeps / 4 Ink Inks / 3 Fly Bomb Fly Bombs 2 Quakey Quakeys and 2 Rock Spider Rock Spiders / 2 Ink Inks and 2 Soy Creep Soy Creeps / 2 Hard Host Hard Hosts 2 Danny Dannies / 2 Gyro Gyros / 2 Gyro Gyros and 1 Bouncer Bouncer / 2 Hardy Hardies 2 Blaster Blasters / 4 Faceless Facelesses / 4 Mole Moles
Ashpit Ashpit 3 Bony Bonies / 6 Rock Spider Rock Spiders / 3 Clickety Clack Clickety Clacks 3 Bone Fly Bone Flies / 3 Flesh Maiden Flesh Maidens / 2 Necro Necros 2 Hardy Hardies / 2 Big Bony Big Bonies / 3 Carrion Princess Carrion Princesss / 4 Dust Dusts 3 Pasty Pasties / 2 Blaster Blasters and 2 Fly Bomb Fly Bombs / 4 Bony Bonies and 3 Dusty Death's Head Dusty Death's Heads / 3 Crackle Crackles
Depths Depths 3 Vis Vises / 4 Baby Babies / 4 Brain Brains 4 Leaper Leapers / 5 Rotty Rotties / 3 Lil' Haunt Lil' Haunts 4 Knight Knights / 3 Fat Sack Fat Sacks / 3 Needle Needles 3 Mr. Maw Mr. Maws / 3 Wizoob Wizoobs / 3 Mom's Hand Mom's Hands / 2 Mr. Red Maw Mr. Red Maws
Necropolis Necropolis 3 Bony Bonies / 3 Gurgle Gurgles / 2 Lil' Haunt Lil' Haunts 2 Swarmer Swarmers and 4 Pooter Pooters / 3 Double Vis Double Vises / 3 Rage Creep Rage Creeps 2 Mask + Heart Mask + Heart Pairs / 4 Keeper Keepers / 3 Psychic Maw Psychic Maws / 3 Eggy Eggies 3 Fat Sack Fat Sacks / 3 Wizoob Wizoobs / 3 Hanger Hangers / 1 Dinga Dingas
Dank Depths Dank Depths 4 Clot Clots / 2 Dank Squirt Dank Squirts / 2 Sticky Leaper Sticky Leapers 6 Dank Death's Head Dank Death's Heads / 1 Mega Clotty Mega Clotty / 4 Slog Slogs / 6 Ink Inks 3 Black Bony Black Bonies and 2 Death's Head Death's Heads / 4 Black Bony Black Bonies / 3 Dank Charger Dank Chargers / 2 Tar Boy Tar Boys and 1 Dank Squirt Dank Squirt 1 Mom's Dead Hand Mom's Dead Hand and 1 Dank Globin Dank Globin / 4 Dank Globin Dank Globins / 2 3-Segment Butt Slicker Butt Slickers / 5 Sticky Leaper Sticky Leapers
Mausoleum Mausoleum 3 Bony Bonies / 2 Psychic Maw Psychic Maws / 4 Soul Sucker Soul Suckers 2 Loose Knight Loose Knights / 2 Whipper Whippers / 4 Candler Candlers / 1 Cursed Globin Cursed Globin 2 Snapper Snappers / 2 Vis Versa Vis Versas / 2 Vis Fatty Vis Fatties / 2 Vis Versa Vis Versas 2 Lil Portal Lil Portals/ 1 Bony Bony and 2 Cultist Cultists / 2 Bishop Bishops and 2 Baby Begotten Baby Begottens / 2 Revenant Revenants
Gehenna Gehenna 3 Bony Bonies / 4 Soul Sucker Soul Suckers | 2 Begotten Begottens / 2 Poofer Poofers 2 Whipper Whippers / 2 Baby Begotten Baby Begottens / 1 Cursed Globin Cursed Globin / 2 Snapper Snappers 2 Maze Roamer Maze Roamers / 2 Vis Versa Vis Versas / 2 Vis Fatty Vis Fatties / 2 Black Knight Black Knights 3 Blood Cultist Blood Cultists / 2 Mask II + 1/2 Heart Mask II + 1/2 Heart Pairs / 8 Goat Goats / 3 Black Goat Black Goats
Womb Womb 3 3-Segment Larry Jr. Larry Jr. / 2 MemBrain MemBrains / 3 Fred Freds 4 Leech Leeches / 3 Swinger Swingers / 2 Mega Clotty Mega Clotties 1 Chub Chub / 2 Monstro Monstros / 1 Fistula Fistula 2 Gemini Geminis / 2 Pin Pins / 2 Polycephalus Polycephaluses
Utero Utero 3 3-Segment The Hollow The Hollows / 2 Mama Guts Mama Guts / 4 Mask + Heart Hearts 2 Oob Oobs / 3 Gurgling Gurglings / 4 Para-Bite Para-Bites / 2 Planetoid Planetoids 1 C.H.A.D. C.H.A.D./ 3 Mask + Heart Mask + Heart Pairs / 2 Widow Widows / 4 Red Ghost Red Ghosts / 2 Floast Floasts 2 Blighted Ovum Blighted Ovums / 1 Gluttony Gluttony and 1 Super Gluttony Super Gluttony / 1 Blastocyst Blastocyst (Small) and 4 Embryo Embryos / 4 Poison Mind Poison Minds/ 2 Peeping Fatty Peeping Fatties
Scarred Womb Scarred Womb 1 Mama Guts Mama Guts and 4 Scarred Guts Scarred Guts / 2 Red Host Red Hosts and 2 Brain Brains / 3 Fistuloid Fistuloids 4 Scarred Double Vis Scarred Double Vises / 4 Scarred Para-Bite Scarred Para-Bites 4 Meatball Meatballs / 3 Flesh Mobile Host Flesh Mobile Hosts 2 Monstro Monstros / 2 Eye Eyes and 4 Nerve Ending Nerve Endings / 2 Leper Lepers (Stage 2) and 2 Leper Lepers (Stage 4)
Corpse Corpse 20 Small Maggot Small Maggots / 2 Sloth Sloths / 2 Level 2 Charger Level 2 Chargers and 4 Charger Chargers / 4 Pustule Pustules 3 Wrinkly Baby Wrinkly Babies / 3 Sick Boom Fly Sick Boom Flies / 2 Level 2 Horf Level 2 Horfs / 3 Twitchy Twitchies 3 Cyst Cysts / 1 Cohort Cohort / 2 Mama Fly Mama Flies and 4 Pustule Pustules / 2 Level 2 Gaper Level 2 Gapers 1 Chub Chub, 2 Charger Chargers, and 5 Small Maggot Small Maggots / 1 Adult Leech Adult Leech and 2 Leech Leechs / 2 Cyst Cysts and 1 Dead Meat Dead Meat / 4 Level 2 Charger Level 2 Chargers
Cathedral Cathedral 3 Holy Leech Holy Leeches / 3 Angelic Baby Angelic Babies / 4 Bony Bonies and 2 Death's Head Death's Heads 5 Wizoob Wizoobs / 7 Pale Fatty Pale Fatties / 4 Hanger Hangers / 3 Candler Candlers 2 Wrath Wraths / 2 Psy Tumor Psy Tumors / 1 Daddy Long Legs Daddy Long Legs / 2 Bishop Bishops and 1 Angelic Baby Angelic Baby / 2 Holy Mulligan Holy Mulligans 1 Conquest Conquest / 1 The Headless Horseman Headless Horsemen Heads / 2 Greed Greeds / 2 Holy Bony Holy Bonies
Sheol Sheol 4 Kamikaze Leech Kamikaze Leeches / 3 Begotten Begottens / 3 Black Globin Black Globins / 3 Imp Imps 3 Selfless Knight Selfless Knights / 2 Black Maw Black Maws / 4 Black Globin's Head Black Globin's Heads / 3 Whipper Whippers 4 Nulls Nulls / 2 Bloodshot Eye Bloodshot Eyes / 2 Camillo Jr. Camillo Jr. / 2 Snapper Snappers / 2 Bloodshot Eye Bloodshot Eyes and 1 Soul Sucker Soul Sucker 2 Greed Greeds / 1 Death Death / 2 Dark One Dark Ones / 1 The Pile The Pile

Boss Challenge Room[]

Boss Challenge Room Door.png

Boss Challenge Rooms can only be entered if Isaac has one or fewer hearts of health. Picking up its contents will cause two waves of bosses to spawn. Starting on Chapter 4, they use the same waves of bosses as Normal Challenge Rooms.

Added in Afterbirth † Each wave cleared will charge Isaac's active item by 1.

Removed in Repentance The Boss Challenge Room door only counts Red Hearts in determining whether Isaac is allowed to enter. Soul Hearts are ignored.
Added in Repentance Boss Challenge Rooms require one or fewer hearts total to enter, counting Soul Hearts. Boss Challenge Rooms will always contain an item from the Boss item pool.

The bosses that spawn depend on the current chapter. Alternate floors have two possible sets of bosses that could spawn:

Removed in Repentance Boss Waves[]

Floor Wave 1 Wave 2
Basement Basement Monstro Monstro Larry Jr. Larry Jr.
Cellar Cellar Pin Pin Gurglings Gurglings
Dingle Dingle Gemini Gemini
Caves Caves Chub Chub Peep Peep
Catacombs Catacombs Mega Fatty Mega Fatty Mega Maw Mega Maw
Fistula Fistula The Haunt The Haunt
Depths Depths Loki Loki Monstro II Monstro II
Necropolis Necropolis Mega Maw Mega Maw The Husk The Husk
Dark One Dark One Loki Loki

Added in Repentance Boss Waves (Sorted By Difficulty)[]

Floor Easy Normal Hard
Basement Basement Monstro Monstro / Gemini Gemini 3 3-Segment Larry Jr. Larry Jr. / The Duke of Flies The Duke of Flies Gurglings Gurglings / Dingle Dingle
Cellar Cellar Pin Pin / The Duke of Flies The Duke of Flies Widow Widow/ Blighted Ovum Blighted Ovum The Haunt The Haunt / Little Horn Little Horn / Baby Plum Baby Plum
Burning Basement Burning Basement Dangle Dangle Turdlings Turdlings / Little Horn Little Horn Rag Man Rag Man
Downpour Downpour The Haunt The Haunt The Rainmaker The Rainmaker Lil Blub Lil Blub / Min-Min Min-Min
Dross Dross Dingle Dingle Lil Blub Lil Blub 5-Segment Turdlet Turdlet/ Clog Clog
Caves Caves Chub Chub / Fistula Fistula Peep Peep / Gurdy Jr. Gurdy Jr. / Pin Pin and 2 Needle Needles Polycephalus Polycephalus / Mega Maw Mega Maw
Catacombs Catacombs The Husk The Husk / 2 4-Segment The Hollow The Hollows The Wretched The Wretched and 2 Trite Trites / Gurdy Jr. Gurdy Jr. / The Stain The Stain Dark One Dark One / Carrion Queen Carrion Queen
Flooded Caves Flooded Caves The Forsaken The Forsaken / Gurdy Jr. Gurdy Jr. The Stain The Stain/ Rag Mega Rag Mega The Frail The Frail / Big Horn Big Horn
Mines Mines 2 Wrath Wraths 4-Segment Larry Jr. Larry Jr., 5-Segment Tuff Twins Tuff Twin, and 1 Bomb Grimace Bomb Grimace Reap Creep Reap Creep
Ashpit Ashpit Super Wrath Super Wrath The Pile The Pile / 5-Segment The Shell The Shell and 1 Bomb Grimace Bomb Grimace Singe Singe and 4 Singe's Ball Singe's Balls / Clutch Clutch
Depths Depths Loki Loki / Gurdy Gurdy Monstro II Monstro II / 2 Little Horn Little Horns The Bloat The Bloat / The Gate The Gate
Necropolis Necropolis Loki Loki The Adversary The Adversary / The Pile The Pile The Cage The Cage / Mask of Infamy Mask of Infamy
Dank Depths Dank Depths Gish Gish / Monstro II Monstro II 2 Little Horn Little Horns / 2 Rag Man Rag Men Brownie Brownie / Sisters Vis Sisters Vis
Mausoleum Mausoleum Rag Mega Rag Mega The Heretic The Heretic The Siren The Siren
Gehenna Gehenna Sisters Vis Sisters Vis The Visage The Visage The Horny Boys The Horny Boys
Womb Womb Blastocyst Blastocyst / Lokii Lokii The Stain The Stain / 3 Gurglings Gurglings The Bloat The Bloat / The Cage The Cage
Utero Utero Blastocyst Blastocyst / Lokii Lokii Teratoma Teratoma / Daddy Long Legs Daddy Long Legs The Bloat The Bloat / 2 Gurdy Jr. Gurdy Jr.s
Scarred Womb Scarred Womb Blastocyst Blastocyst / Polycephalus Polycephalus / Lokii Lokii Fistula Fistula / Gurdy Jr. Gurdy Jr. + 1 Gurglings Gurgling The Matriarch The Matriarch / The Bloat The Bloat / Mama Gurdy Mama Gurdy
Corpse Corpse 2 Super Sloth Super Sloths / 2 Super Gluttony Super Gluttonies Chimera Chimera / 2 Chub Chubs The Scourge The Scourge

Layouts[]

The following room maps were made using Basement Renovator for cleaner viewing; note that both normal and boss challenge rooms use the same layouts.

ID Weight Notes Image preview
0-2 1.0 This room appears three times in the room pool, making it three times more likely to appear than normal. Challenge 0-2.png
3-4 1.0 This room appears twice in the room pool, making it twice as likely to appear than normal. Challenge 3-4.png
5 0.5 The item will be from the Treasure Room pool. Challenge 5.png
6 1.0 Challenge 6.png
7 0.75 Challenge 7.png
8 0.5 Challenge 8.png
9 0.5 Challenge 9.png
10 0.5 Challenge 10.png
11 1.0 Challenge 11.png
12 1.0 Challenge 12.png

Notes[]

  • Added in RepentanceTemporary Tattoo Temporary Tattoo will cause an additional chest to spawn after a normal Challenge Room is completed as well as cause an additional item from the Boss item pool to spawn after completing a Boss Challenge Room.
  • Added in Afterbirth The waves appear in random orders rather than the orders listed above.
  • If Isaac has some way to teleport out of the room, the contents of the Challenge Room can be taken without fighting the enemies.
    • If a trigger to start a challenge room is left in the room after teleporting out (such as a chest or active item) or an item is spawned in the room and collected (such as rewards from Beggar.png Beggars or Blood Donation Machine.png Blood Donation Machines), the challenge room will start over when it is picked up/opened.
  • If a Secret Room Secret Room is next to a Challenge Room, it can be entered by using a bomb in the Secret Room on the wall separating the two rooms. However, the entrance to the Secret Room from the Challenge Room closes itself, meaning Isaac cannot escape through the Secret Room entrance.
    • Added in Repentance If Isaac bombs into either variation of Challenge Room through a Secret Room and leaves, the bombed entrance will close until and must be bombed open again.
  • If Spiders spawn from a Red Chest before or during a wave, the Spiders have to be killed in order for the waves to start/continue.
    • If Spiders spawn from a Red Chest after the challenge room is cleared, Isaac can leave the room without fighting them.
  • Because of the health requirements, both types of Challenge Room will always be open for The Lost The Lost Removed in Repentanceand ??? ???.
    • Added in Repentance As Boss Challenge Rooms now take soul hearts into account, Soul Heart characters are now also forced to be at one heart before being allowed access.
  • Doors to challenge rooms can be opened regardless of health with Dad's Key Dad's Key or Added in Afterbirth †Mr. ME! Mr. ME!
  • Added in RepentanceUsing Added in AfterbirthGlowing Hour Glass Glowing Hour Glass will re-roll the bosses in the room.

Bugs[]

Bug Bug! Removed in Afterbirth † If the game crashes or you close it before you finish the challenge room it will crash every time you try to continue that run.
Bug Bug! If Isaac encounters Greed or Super Greed in a Secret Room next to a boss challenge room, the path to the boss challenge room is boarded up from the mini-boss fight. Defeating Greed or Super Greed does not remove the boards, despite the room no longer being active.


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