Challenge Room

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Challenge Rooms are special rooms that allow Isaac to fight enemies for extra rewards. They will only spawn on a floor if, upon entering the floor, Isaac's total health is greater than or equal to his number of heart containers. There are two kinds of challenge rooms: Normal Challenge Rooms, which appear on the first floor of a chapter or on XL floors, and Boss Challenge Rooms, which appear on the second floor of a chapter.

Normal Challenge Room[edit | edit source]

Challenge Room.png

Normal Challenge Rooms can only be entered if Isaac's total health (Red, Black, and Soul Hearts) is greater than or equal to his number of heart containers. Picking up its contents will cause three waves of enemies to spawn. While in chapter 4 (The Womb The Womb/Utero Utero), 3 waves of bosses will be spawned (usually bosses from The Basement The Basement/The Caves The Caves).

Added in Afterbirth † Each wave cleared will charge Isaac's active item by 1.

Removed in Repentance Waves[edit | edit source]

The enemies that spawn depend on the current chapter, and each chapter has two different possible sets of enemies:

Floor Wave 1 Wave 2 Wave 3
The Basement The Basement 3 Hoppers 3 Horfs 2 Clotties
5 Attack Flies 5 Pooters 4 Gapers
The Cellar The Cellar 3 Fattys 3 Round Worms 5 Dips
3 Gapers 3 Horfs 3 Fatties
The Caves The Caves 5 Chargers 4 Globins 4 Maws
5 Spitties 3 Hives 3 Hosts
The Catacombs The Catacombs 2 Bonies 2 Tumors 3 Wall Creeps
4 Round Worms 3 Nightcrawlers 1 Grub
The Depths The Depths 3 Vis 4 Leapers 4 Globins
4 Babies 5 Hoppers 4 Knights
Necropolis Necropolis 4 Knights 3 Mom's Hands 3 Fat Sacks
4 Bonies 2 Squirts 3 Nulls
The Womb The Womb
Utero Utero
3 Larry Jrs Chub and 3 Chargers 2 Monstros
Fistula Loki Chub
Cathedral Cathedral
Sheol Sheol
3 Larry Jrs Chub and 3 Chargers 2 Monstros
Fistula Loki Chub

Added in Repentance Waves (done in Hard Mode)[edit | edit source]

Floor Wave 1 Wave 2 Wave 3
Downpour Downpour 2 Preys/4 Frowning Gapers and 1 Wraith 3 Bloaties 2 Mullighouls
Dross Dross 3 Cloggies 4 Blurbs 2 Cloggies and 1 Splurt
The Caves The Caves 4 Chargers/3 Mulligans/3 Globins 2 Conjoined Fatties/3 Hosts/3 Maws 4 Pale Fatties/2 Tumors/4 Red Boom Flies
The Catacombs The Catacombs 3 Boom Flies/Buttlickers/3 Night Crawlers 1 Grub/3 Psychic Horfs/3 One Tooths 2 Walking Hosts/4 Gurgles/4 Chubbers
Flooded Caves Flooded Caves 2 Drowned Hives 2 Drowned Boom Flies and 2 Dukies 4 Blubbers and 2 Conjoined Fatties
Mines Mines 2 Quakeys and 2 Rock Spiders/2 Inks and 2 Soy Creeps 2 Gyros/2 Gyros and 1 Bouncer/2 Dannies 4 Faceless/4 Moles
Ashpit Ashpit 3 Bone Flies 3 Carrion Princesses 4 Bonies and 3 Dusty Death's Head
The Depths The Depths 4 Leapers/3 Lil' Haunts/3 Needles/3 Night Crawlers/3 Rotties 3 Fat Sacks/3 Needles/4 Knights 3 Wizoobs/3 Mom's Hands/3 Mr. Maws/2 Mr. Red Maws
Necropolis Necropolis 3 Double Vises/2 Swarmers and 4 Pooters/3 Rage Creeps 3 Eggys/4 Keepers/2 Mask + Hearts 3 Fat Sacks/3 Wizoobs/1 Dinga/3 Hangers
Mausoleum Mausoleum 4 Candlers/2 Whippers/2 Loose Knights 2 Snappers/2 Vis Versa 2 Lil Portals/1 Bony and 2 Cultists
Gehenna Gehenna 1 Cursed Globin 2 Vis Fatties and 2 Fetal Demons 2 Mask II + 1/2 Hearts and 1 1/2 Heart
The Womb The Womb 3 Leeches/2 Mega Clotties/3 Swingers Fistula/1 Chub/2 Monstros 2 Pins/Gluttony and Super Gluttony/2 Polycephalus
Utero Utero 4 Para-Bites/2 Planetoids/3 Gurglings/2 Oobs 1 C.H.A.D/4 Red Ghosts/2 Floating Hosts/3 Mask + Hearts 2 Blighted Ovums/4 Brains/2 Peeping Fatties/Gluttony and Super Gluttony
Corpse Corpse 2 Level 2 Horfs/3 Twitchies 3 Cysts/2 Level 2 Gapers 2 Leeches and 1 Adult Leech/2 Cysts and 1 Dead Meat/4 Level 2 Chargers
Cathedral Cathedral 3 Candlers 2 Wraths 2 Greeds
Sheol Sheol 4 Black Globin's Heads/3 Selfless Knights/3 Whippers 2 Bloodshot Eyes/2 Bloodshot Eyes and 1 Soul Sucker/2 Camilio Jr.s/2 Snappers 2 Greeds/1 Death/2 Dark Ones/The Pile

Boss Challenge Room[edit | edit source]

Boss Challenge Room Door.png

Boss Challenge Rooms can only be entered if Isaac has one or less heart of health. Picking up its contents will cause two waves of bosses to spawn. Starting on Chapter 4, they use the same waves of bosses as Normal Challenge Rooms.

Added in Afterbirth † Each wave cleared will charge Isaac's active item by 1.

Removed in Repentance The Boss Challenge Room door only counts Red Hearts in determining whether Isaac is allowed to enter. Soul Hearts are ignored.
Added in Repentance Boss Challenge Rooms require one or less health total to enter, counting Soul Hearts. Boss Challenge Rooms will always contain an item from the Boss item pool.

The bosses that spawn depend on the current chapter. Alternate floors have two possible sets of bosses that could spawn:

Removed in Repentance Boss Waves[edit | edit source]

Floor Wave 1 Wave 2
The Basement The Basement Monstro Larry Jr.
The Cellar The Cellar Pin Gurglings
Dingle Gemini
The Caves The Caves Chub Peep
The Catacombs The Catacombs Mega Fatty Mega Maw
Fistula The Haunt
The Depths The Depths Loki Monstro II
Necropolis Necropolis Mega Maw The Husk
Dark One Loki

Added in Repentance Boss Waves (done in Hard Mode)[edit | edit source]

Floor Wave 1 Wave 2
The Basement The Basement The Duke of Flies/Larry Jr. Dingle/Gurglings
The Cellar The Cellar Widow Baby Plum/The Haunt
Burning Basement Burning Basement Turdlings/Little Horn Rag Man
Downpour Downpour The Rainmaker Lil Blub/Min-Min
Dross Dross Lil Blub Turdlet
The Caves The Caves Gurdy Jr./2 Needles, 1 Pin/Peep Mega Maw/Polycephalus
The Catacombs The Catacombs Gurdy Jr./Peep/The Wretched Carrion Queen/Dark One/Mega Maw
Flooded Caves Flooded Caves Rag Mega Big Horn
Mines Mines Larry Jr. and 1 Tuff Twin Reap Creep
Ashpit Ashpit The Pile The Shell
The Depths The Depths The Duke of Flies/Gurdy Jr./Little Horn/Monstro II The Bloat/Mega Maw/Polycephalus
Necropolis Necropolis 2 Little Horns/The Pile/The Stain The Cage/Dark One
The Womb The Womb 3 Gurglings/The Stain The Bloat/The Cage
Utero Utero Daddy Long Legs/Teratoma The Bloat/2 Gurdy Jr.s

Layouts[edit | edit source]

The following room maps were made using Basement Renovator for cleaner viewing; note that both normal and boss challenge rooms use the same layouts.

ID Weight Notes Image preview
0-2 1.0 This room appears three times in the room pool, making it three times more likely to appear than normal. Challenge 0-2.png
3-4 1.0 This room appears twice in the room pool, making it twice as likely to appear than normal. Challenge 3-4.png
5 0.5 The item will be from the Treasure Room pool. Challenge 5.png
6 1.0 Challenge 6.png
7 0.75 Challenge 7.png
8 0.5 Challenge 8.png
9 0.5 Challenge 9.png
10 0.5 Challenge 10.png
11 1.0 Challenge 11.png
12 1.0 Challenge 12.png

Notes[edit | edit source]

  • Added in Afterbirth The waves appear in random orders rather than the orders listed above.
  • If Isaac has some way to teleport out of the room, the contents of the Challenge Room can be taken without fighting the enemies.
    • If a trigger to start a challenge room is left in the room after teleporting out (such as a chest or active item) or an item is spawned in the room and collected (such as rewards from Beggars or Blood Donation Machines), the challenge room will start over when it is picked up/opened.
  • If a Secret Room is next to a Challenge Room, it can be entered by using a bomb in the Secret Room on the wall separating the two rooms. However, the entrance to the Secret Room from the Challenge Room closes itself, meaning Isaac cannot escape through the Secret Room entrance.
  • If Spiders spawn from a Red Chest before or during a wave, the Spiders have to be killed in order for the waves to start/continue.
    • If Spiders spawn from a Red Chest after the challenge room is cleared, the player can leave the room without fighting them.
  • If a Secret Room is next to a Boss Challenge Room, it can be entered by using a bomb in the Secret Room on the wall separating the two rooms. However, the entrance to the Secret Room from the Boss Challenge Room closes itself, meaning Isaac cannot escape through the Secret Room entrance.
  • Because of the health requirements, both types of Challenge Room will always be open for ???Removed in Repentanceand The Lost.

Bugs[edit | edit source]

Bug Bug! Removed in Afterbirth † If the game crashes or you close it before you finish the challenge room it will crash every time you try to continue that run.


SmallIsaac.png Rooms SmallIsaac.png

Unlocked Added in Afterbirth Added in Afterbirth †
Boss Room Icon.png
Boss Room
Mini-Boss Room Icon.png
Mini-Boss Room
Curse Room Icon.png
Curse Room
Sacrifice Room Icon.png
Sacrifice Room
Room 1x1 small.png
Closet
Room 1x1.png
Grave Room
Locked Added in Repentance
Treasure Room Icon.png
Treasure Room
Shop Icon.png
Shop
Library Icon.png
Library
Planetarium Icon.png
Planetarium
Slotted Arcade Icon.png
Arcade
Barred Challenge Room Icon.png
Challenge Room
Boss Challenge Room Icon.png
Boss Challenge Room
Double-locked Vault Icon.png
Vault
Dice Room Icon.png
Dice Room
Double-boarded Bedroom Icon.png Bedroom2 Icon.png
Bedroom
Hidden Added in Repentance
Secret Room Icon.png
Secret Room
Super Secret Room Icon.png
Super Secret Room
Super Secret Room Icon.png
Crawl Space
Super Secret Room Icon.png
Black Market
Super Secret Room Icon.png
I AM ERROR
Ultra Secret Room Icon.png
Ultra Secret Room
Post-boss Added in Afterbirth
Devil Room Icon.png
Devil Room
Angel Room Icon.png
Angel Room
Super Secret Room Icon.png
Boss Rush
Super Secret Room Icon.png
Blue Womb Entrance
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