Binding of Isaac: Rebirth Wiki
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Binding of Isaac: Rebirth Wiki

Challenge Rooms are special rooms that allow Isaac to fight enemies for extra rewards. They will only spawn on a floor if, upon entering the floor, Isaac's total health is greater than or equal to his number of heart containers. There are two kinds of challenge rooms: Normal Challenge Rooms, which appear on the first floor of a chapter or on XL floors, and Boss Challenge Rooms, which appear on the second floor of a chapter.

Normal Challenge Room[]

Challenge Room.png

Normal Challenge Rooms can only be entered if Isaac's total health (Red, Black, and Soul Hearts) is greater than or equal to his number of heart containers. Picking up its contents will cause three waves of enemies to spawn. While in chapter 4 (Womb Womb/Utero Utero/Scarred Womb Scarred Womb), 3 waves of bosses will be spawned, typically from Chapters 1 or 2.

Added in Afterbirth † Each wave cleared will charge Isaac's active item by 1.

Removed in Repentance Waves[]

The enemies that spawn depend on the current chapter, and each chapter has two different possible sets of enemies:

Floor Wave 1 Wave 2 Wave 3
Basement Basement 3 Hoppers 3 Horfs 2 Clotties
5 Attack Flies 5 Pooters 4 Gapers
Cellar Cellar 3 Fatties 3 Round Worms 5 Dips
3 Gapers 3 Horfs 3 Fatties
Caves Caves 5 Chargers 4 Globins 4 Maws
5 Spitties 3 Hives 3 Hosts
Catacombs Catacombs 2 Bonies 2 Tumors 3 Wall Creeps
4 Round Worms 3 Nightcrawlers 1 Grub
Depths Depths 3 Vis 4 Leapers 4 Globins
4 Babies 5 Hoppers 4 Knights
Necropolis Necropolis 4 Knights 3 Mom's Hands 3 Fat Sacks
4 Bonies 2 Squirts 3 Nulls
Womb Womb
Utero Utero
3 Larry Jrs Chub and 3 Chargers 2 Monstros
Fistula Loki Chub
Cathedral Cathedral
Sheol Sheol
3 Larry Jrs Chub and 3 Chargers 2 Monstros
Fistula Loki Chub

Added in Repentance Waves (Sorted By Difficulty; Basement, Cellar, and Burning Basement Unused)[]

Floor Easy Normal Hard Hardest
Basement Basement 3 Hoppers / 5 Attack Flies / 4 Dips 3 Horfs / 5 Pooters / 2 3-Segment Army Flies 2 Clotties / 4 Frowning Gapers / 1 Squirt and 2 Dips 3 Level 2 Flies and 2 Full Flies / 3 Suckers / 4 Cyclopias / 3 Ministros
Cellar Cellar 3 Level 2 Spiders / 5 Spiders / 3 Sacks 4 Trites / 3 Small Crazy Long Legs / 3 Wall Creeps 2 Nests / 2 Crazy Long Legs / 3 Small Baby Long Legs 2 Baby Long Legs / 2 Big Spiders and 2 Spiders / 2 Blind Creeps / 2 Migraines
Burning Basement Burning Basement 2 Flaming Hoppers 5 Dart Flies / 2 Pairs of 5 Swarm Flies / 2 Pairs of 4 Swarm Spiders 3 Flaming Fatties / 4 Suckers / 2 Grilled Clotties 4 Flaming Hoppers / 4 Crispies
Downpour Downpour 2 Blurbs / 5 Small Leeches / 4 Striders 4 Frowning Gapers and 1 Wraith / 2 Preys / 3 Sub Horfs 5 Willos and 2 Striders / 2 Bubbles and 2 Striders / 3 Bloaties 4 Sub Horfs and 2 Blurbs / 2 Mullighouls
Dross Dross 5 Drips / 5 Small Leeches / 2 Dump Heads 3 Cloggies / 2 Fartigans and 4 Gas Clouds / 4 Big Corns 4 Blurbs / 2 Bubbles and 2 Sub Horfs / 3 Dumps 2 Cloggies and 1 Splurt / 2 Fartigans and 2 Poot Mines
Caves Caves 5 Conjoined Spitties / 4 Boils / 6 Maggots 3 Hives / 3 Globins / 4 Chargers 3 Hosts / 3 Maws / 2 Conjoined Fatties 4 Pale Fatties/ 2 Tumors / 4 Red Boom Flies
Catacombs Catacombs 5 Round Worms / 4 Wall Creeps / 4 Splashers 3 Night Crawlers / 2 2-Segment Buttlickers and 1 3-Segment Buttlickers / 3 Boom Flies 1 3-Segment Grub / 3 One Tooths / 3 Psychic Horfs 2 Mobile Hosts / 4 Chubbers / 4 Gurgles
Flooded Caves Flooded Caves 4 Conjoined Spitties / 3 Drowned Boom Flies 2 Drowned Hives / 3 Globins / 3 Night Crawlers 4 Maws / 2 Squirts / 2 Drowned Boom Flies and 2 Dukies 4 Blubbers and 2 Conjoined Fatties / 5 Drowned Chargers / 3 Tube Worms
Mines Mines 4 Soy Creeps / 4 Inks / 3 Fly Bombs 2 Quakeys and 2 Rock Spiders / 2 Inks and 2 Soy Creeps / 2 Hard Hosts 2 Dannies / 2 Gyros / 2 Gyros and 1 Bouncer / 2 Hardies 2 Blasters / 4 Facelesses / 4 Moles
Ashpit Ashpit 3 Bonies / 6 Rock Spiders / 3 Clickity Clacks 3 Bone Flies / 3 Flesh Maidens / 2 Necros 2 Hardies / 3 Big Bonies / 3 Carrion Princesses / 4 Dusts 3 Pasties / 2 Blasters and 2 Fly Bombs / 4 Bonies and 3 Dusty Death's Heads / 3 Crackles
Depths Depths 3 Vises / 4 Babies / 4 Brains 4 Leapers / 5 Rotties / 3 Lil' Haunts 4 Knights / 3 Fat Sacks / 3 Needles 3 Mr. Maws / 3 Wizoobs / 3 Mom's Hands / 2 Mr. Red Maws
Necropolis Necropolis 3 Bonies / 3 Gurgles / 2 Lil' Haunts 2 Swarmers and 4 Pooters / 3 Double Vises / 3 Rage Creeps 2 Mask + Heart Pairs / 4 Keepers / 3 Psychic Maws / 3 Eggies 3 Fat Sacks / 3 Wizoobs / 3 Hangers / 1 Dingas
Dank Depths Dank Depths 4 Clots / 2 Dank Squirts / 2 Sticky Leapers 6 Dank Death's Heads / 1 Mega Clotty / 4 Slogs / 6 Inks 3 Black Bonies and 2 Death's Heads / 4 Black Bonies / 3 Dank Chargers / 2 Tar Boys and 1 Dank Squirt 1 Mom's Dead Hand and 1 Dank Globin / 4 Dank Globins / 2 3-Segment Butt Slickers / 5 Sticky Leapers
Mausoleum Mausoleum 3 Bonies / 2 Psychic Maws / 4 Soul Suckers 2 Loose Knights / 2 Whippers / 4 Candlers / 1 Cursed Globin 2 Snappers / 2 Vis Versas / 2 Vis Fatties

/2 Vis Versas

2 Lil Portals/1 Bony and 2 Cultists / 2 Bishops and 2 Baby Begottens / 2 Revenants
Gehenna Gehenna 3 Bonies / 4 Soul Suckers | 2 Begottens / 2 Poofers 2 Whippers / 2 Baby Begottens / 1 Cursed Globin / 2 Snappers 2 Maze Roamers / 2 Vis Versas / 2 Vis Fatties / 2 Black Knights 3 Blood Cultists / 2 Mask II + 1/2 Hearts Pairs / 8 Goats / 3 Black Goats
Womb Womb 3 3-Segment Larry Jr.s / 2 MemBrains / 3 Freds 4 Leeches / 3 Swingers / 2 Mega Clotties 1 Chub / 2 Monstros / 1 Fistula 2 Geminis / 2 Pins / 2 Polycephaluses
Utero Utero 3 3-Segment The Hollows / 2 Mama Guts / 4 Hearts 2 Oobs / 3 Gurglings / 4 Para-Bites/ 2 Planetoids 1 C.H.A.D/ 3 Mask + Heart Pairs / 2 Widows / 4 Red Ghosts/2 Floating Hosts 2 Blighted Ovums / 1 Gluttony and 1 Super Gluttony / 1 Blastocyst (Small) and 4 Embryos / 4 Poison Minds/2 Peeping Fatties
Scarred Womb Scarred Womb 1 Mama Guts and 4 Scarred Guts / 2 Red Hosts and 2 Brains / 3 Fistuloids 4 Scarred Double Vises / 4 Scarred Para-Bites 4 Meatballs / 3 Flesh Mobile Hosts 2 Monstros / 2 Eyes and 4 Nerve Endings / 2 Lepers (Stage 2) and 2 Lepers (Stage 4)
Corpse Corpse 20 Small Maggots / 2 Sloths / 2 Level 2 Chargers and 4 Chargers / 4 Pustules 3 Wrinkly Babies / 3 Sick Boom Flies / 2 Level 2 Horfs / 3 Twitchies 3 Cysts/ 1 Cohort / 2 Mama Flies and 4 Pustules / 2 Level 2 Gapers 1 Chub, 2 Chargers, and 5 Small Maggots / 1 Adult Leech and 2 Leeches / 2 Cysts and 1 Dead Meat/4 Level 2 Chargers
Cathedral Cathedral 3 Holy Leeches / 3 Angelic Babies / 4 Bonies and 2 Death's Heads 5 Wizoobs / 7 Pale Fatties / 4 Hangers / 3 Candlers 2 Wraths / 2 Psy Tumors / 1 Daddy Long Legs / 2 Bishops and 1 Angelic Baby / 2 Holy Mulligans 1 Conquest / 1 Headless Horsemen Heads / 2 Greeds / 2 Holy Bonies
Sheol Sheol 4 Kamikaze Leeches / 3 Begottens / 3 Black Globins / 3 Imps 3 Selfless Knights / 2 Black Maws / 4 Black Globin's Heads / 3 Whippers 4 Nulls / 2 Bloodshot Eyes / 2 Camilio Jr.s / 2 Snappers / 2 Bloodshot Eyes and 1 Soul Sucker 2 Greeds / 1 Death / 2 Dark Ones / 1 The Pile

Boss Challenge Room[]

Boss Challenge Room Door.png

Boss Challenge Rooms can only be entered if Isaac has one or fewer hearts of health. Picking up its contents will cause two waves of bosses to spawn. Starting on Chapter 4, they use the same waves of bosses as Normal Challenge Rooms.

Added in Afterbirth † Each wave cleared will charge Isaac's active item by 1.

Removed in Repentance The Boss Challenge Room door only counts Red Hearts in determining whether Isaac is allowed to enter. Soul Hearts are ignored.
Added in Repentance Boss Challenge Rooms require one or fewer hearts total to enter, counting Soul Hearts. Boss Challenge Rooms will always contain an item from the Boss item pool.

The bosses that spawn depend on the current chapter. Alternate floors have two possible sets of bosses that could spawn:

Removed in Repentance Boss Waves[]

Floor Wave 1 Wave 2
Basement Basement Monstro Larry Jr.
Cellar Cellar Pin Gurglings
Dingle Gemini
Caves Caves Chub Peep
Catacombs Catacombs Mega Fatty Mega Maw
Fistula The Haunt
Depths Depths Loki Monstro II
Necropolis Necropolis Mega Maw The Husk
Dark One Loki

Added in Repentance Boss Waves (Sorted By Difficulty)[]

Floor Easy Normal Hard
Basement Basement Monstro / Gemini 3 3-Segment Larry Jr. / The Duke of Flies Gurglings / Dingle
Cellar Cellar Pin / The Duke of Flies Widow/Blighted Ovum The Haunt / Little Horn / Baby Plum
Burning Basement Burning Basement Dangle Turdlings / Little Horn Rag Man
Downpour Downpour The Haunt The Rainmaker Lil Blub / Min-Min
Dross Dross Dingle Lil Blub 5-Segment Turdlet/Clog
Caves Caves Chub / Fistula Peep / Gurdy Jr. / Pin and 2 Needles Polycephalus / Mega Maw
Catacombs Catacombs The Husk / 2 4-Segment The Hollows The Wretched and 2 Trites / Gurdy Jr. / The Stain Dark One / Carrion Queen
Flooded Caves Flooded Caves The Forsaken / Gurdy Jr. The Stain/Rag Mega The Frail / Big Horn
Mines Mines 2 Wraths 4-Segment Larry Jr., 5-Segment Tuff Twin, and 1 Bomb Grimace Reap Creep
Ashpit Ashpit Super Wrath The Pile Singe and 4 Singe's Balls / 5-Segment The Shell and 1 Bomb Grimace
Depths Depths Loki / Gurdy Monstro II / 2 Little Horns The Bloat/The Gate
Necropolis Necropolis Loki The Adversary/The Pile The Cage/Mask of Infamy
Dank Depths Dank Depths Gish / Monstro II 2 Little Horns/2 Rag Men Brownie/Sisters Vis
Mausoleum Mausoleum Rag Mega The Heretic The Siren
Gehenna Gehenna Sisters Vis The Visage The Horny Boys
Womb Womb Blastocyst / Lokii The Stain / 3 Gurglings The Bloat/The Cage
Utero Utero Blastocyst / Lokii Teratoma / Daddy Long Legs The Bloat/2 Gurdy Jr.s
Scarred Womb Scarred Womb Blastocyst / Polycephalus / Lokii Fistula/ Gurdy Jr. + 1 Gurgling The Matriarch / The Bloat / Mama Gurdy
Corpse Corpse 2 Super Sloths / 2 Super Gluttonies Chimera / 2 Chubs The Scourge

Layouts[]

The following room maps were made using Basement Renovator for cleaner viewing; note that both normal and boss challenge rooms use the same layouts.

ID Weight Notes Image preview
0-2 1.0 This room appears three times in the room pool, making it three times more likely to appear than normal. Challenge 0-2.png
3-4 1.0 This room appears twice in the room pool, making it twice as likely to appear than normal. Challenge 3-4.png
5 0.5 The item will be from the Treasure Room pool. Challenge 5.png
6 1.0 Challenge 6.png
7 0.75 Challenge 7.png
8 0.5 Challenge 8.png
9 0.5 Challenge 9.png
10 0.5 Challenge 10.png
11 1.0 Challenge 11.png
12 1.0 Challenge 12.png

Notes[]

  • Added in RepentanceTemporary Tattoo Temporary Tattoo will cause an additional chest to spawn after a normal Challenge Room is completed as well as cause an additional item from the Boss item pool to spawn after completing a Boss Challenge Room.
  • Added in Afterbirth The waves appear in random orders rather than the orders listed above.
  • If Isaac has some way to teleport out of the room, the contents of the Challenge Room can be taken without fighting the enemies.
    • If a trigger to start a challenge room is left in the room after teleporting out (such as a chest or active item) or an item is spawned in the room and collected (such as rewards from Beggars or Blood Donation Machines), the challenge room will start over when it is picked up/opened.
  • If a Secret Room is next to a Challenge Room, it can be entered by using a bomb in the Secret Room on the wall separating the two rooms. However, the entrance to the Secret Room from the Challenge Room closes itself, meaning Isaac cannot escape through the Secret Room entrance.
    • Added in Repentance If Isaac bombs into either variation of Challenge Room through a Secret Room and leaves, the bombed entrance will close until and must be bombed open again.
  • If Spiders spawn from a Red Chest before or during a wave, the Spiders have to be killed in order for the waves to start/continue.
    • If Spiders spawn from a Red Chest after the challenge room is cleared, Isaac can leave the room without fighting them.
  • Because of the health requirements, both types of Challenge Room will always be open for The Lost The Lost Removed in Repentanceand ??? ???.
    • Added in Repentance As Boss Challenge Rooms now take soul hearts into account, Soul Heart characters are now also forced to be at one heart before being allowed access.
  • Doors to challenge rooms can be opened regardless of health with Dad's Key Dad's Key or Added in Afterbirth †Mr. ME! Mr. ME!

Bugs[]

Bug Bug! Removed in Afterbirth † If the game crashes or you close it before you finish the challenge room it will crash every time you try to continue that run.
Bug Bug! If Isaac encounters Greed or Super Greed in a Secret Room next to a boss challenge room, the path to the boss challenge room is boarded up from the mini-boss fight. Defeating Greed or Super Greed does not remove the boards, despite the room no longer being active.


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