The Cathedral is one of two unlockable floors following Womb/ Utero/ Scarred Womb, the other being Sheol. After defeating Mom's Heart, an Angel Room may spawn containing a beam of light leading upwards to the Cathedral. Unlocking and defeating It Lives will always spawn a beam of light leading to the Cathedral, as well as a trapdoor leading to Sheol.
The Cathedral has only one floor, featuring mostly undead and/or holy enemies, and the boss is always Isaac.
||38.1.0||Slowly floats towards Isaac and fires three spread shots. Occasionally teleports and reappears elsewhere.|
||227.0||Wanders around the room and throws a bone at Isaac when he is in sight.|
||212.0||Invulnerable enemy that moves diagonally around the room, dealing contact damage. Dies when the room is cleared.|
||15.1||Randomly wanders around the room, firing 4 tar shots in the ordinal directions.|
||208.2||Slowly walks towards Isaac. Occasionally farts and shoots 6 blood shots in all directions. Has a chance to turn into a Blubber or Half Sack upon death.|
||209.0||Slowly walks towards Isaac. Occasionally leaps off-screen and attempts to land on Isaac, leaving a puddle of Red Creep and shooting 6 blood shots in all directions where it lands. Has a chance to turn into a Blubber or Half Sack upon death.|
||254.0||Behaves like the Knight but slower, and can fly over all obstacles.|
||24.1||Quickly chases Isaac, dealing a full heart of contact damage. Upon taking enough damage, it collapses into a pile of goo, which will slide away from Isaac and can be killed. Will regenerate after a short period of time if not killed quickly.|
||90.0||Floats around the room, firing 3 spread blood shots at Isaac. Upon damaging Isaac, Isaac drops 2 coins on the ground but loses 3. Spawns with an Eternal Fly circling around it.|
||86.0||Hops around the room, firing 2 blood shots in a V formation at Isaac. Upon damaging Isaac, Isaac drops 2 coins on the ground but loses 3.|
||55.0||Quickly charges towards Isaac when he gets in its line of sight.|
||55.2||Quickly charges towards Isaac when he gets in its line of sight. Explodes upon death. Spawns with an Eternal Fly circling it, which instantly dies when the Holy Leech explodes.|
||213.0||Falls from the ceiling. If it lands on Isaac, it deals damage and teleports the player back to the spawn room of the floor. Entering a room containing Mom's Hand will warn the player with Mom's laugh.|
||246.0||Quickly leaps towards Isaac and deals contact damage. Randomly shoots two purple shots that orbit around it.|
||226.1||Slowly walks towards Isaac, leaving short-lasting Red Creep behind itself. Spawns a Bony or two Attack Flies upon death.|
||61.1||Flies slowly towards Isaac, firing an Ipecac shot towards Isaac upon death.|
||253.0||Moves around the room diagonally, occasionally firing 6 homing shots in all directions. Fires more often upon getting damaged.|
||248.0||Stationary enemy that fires a homing shot at Isaac when he gets in range of them.|
||26.2||Slowly floats towards Isaac, firing homing shots at him when he is in their range. Spawns with an Eternal Fly orbiting around it.|
||219.0||Disappears, then appears near Isaac and spits a cluster of spectral tears at him.|
||260.0||Floats toward Isaac and occasionally fires a blood shot at him, predicting his movements.|
||44.1||Quickly slides towards Isaac when he gets in its line of sight, then moves slowly back to its original position. Cannot be destroyed.|
||42.0||Stationary and invulnerable monster that occasionally fires a blood shot at Isaac. Stops firing when room is cleared.|
|Cross Stone Shooter
||202.10||Stationary and invulnerable monster that continuously fires shots in four directions, alternating between cardinal and ordinal.|
||60.2||Rapidly summons several beams of light around Isaac every few seconds.|
||22.2||Wanders around the room, avoiding Isaac and spawning Small Angelic Babies if he gets close. Spawns 1-2 Embryos upon death.|
||805.0||Shields all enemies in the room, preventing them from taking damage. Fires shots in the cardinal directions when it takes damage.|
||227.1||Wanders around the room, throwing a bone at Isaac when he is in sight. The thrown bone leaves behind a blue flame that deals contact damage, blocks Isaac’s shots, and despawns after some time.|
||817.1||Wanders around the room, avoiding Isaac and spawning Willos if he gets close. Spawns 1-2 Willos upon death.|
- Some rooms contain Angel Statues that can be bombed to summon the Angels and acquire key pieces.
- If a trapdoor appears, it will lead to The Chest.
- In the original Binding of Isaac, the windows along the walls were much larger and had different patterns resembling Isaac and a crucifix.
- One of the interpretations of this floor's theme Everlasting Hymn includes the sentence "Harm yourself and God will grant you strength" many consider this translation "Too extreme" or somehow having no parallels with this game's story, however all the original endings (based on the items Isaac collects) end with Isaac finding several objects and using them to harm himself, which indeed does increase his power in-game
The music for the Cathedral is titled Everlasting Hymn.
|Chapter 1||Chapter 2||Chapter 3||Chapter 4||Optional||Chapter 5||Chapter 6||Endgame|
|The Basement||The Caves||The Depths||The Womb|
|The Cellar||The Catacombs||Necropolis||Utero||???||The Void|
|Burning Basement||Flooded Caves||Dank Depths||Scarred Womb|
|The Basement||The Caves||The Depths||The Womb||Sheol||The Shop||Ultra Greed|
|The Binding of Isaac: Rebirth|
|Achievements||Attributes||Bosses||Cards and Runes||Challenges||Chapters|