Binding of Isaac: Rebirth Wiki

Added in Afterbirth

Betrayal is an unlockable passive item added in The Binding of Isaac: Afterbirth.


  • Upon taking damage, all enemies in the room are charmed.
    • Added in Afterbirth † It won't trigger if there are less than 2 enemies in the room.


This item is extremely strong against the second and third phase of ??? ???, as its own eternal flies become charmed and deal massive damage to the boss, often killing it after even just two uses.

In-game Footage[]


  • This is a reference to Judas betraying Jesus after the Last Supper, leading to Jesus' death by crucifixion.


PC Greed mode only CA8H WX1Q (First Golden Treasure Room)

PC JRJH 8HPW (Treasure Room adjacent to spawn; 1 room down on Hard mode)

PC Normal mode only FNQE 4P7W (Treasure Room adjacent to spawn)

Switch Greedier mode only 8VJ6 FPC3 (First Golden Treasure Room)