1up! is a passive item.
- Gives Isaac an extra life. Upon death, he will respawn in the most recent non-hostile room with full health, after which 1up! will disappear.
- If Isaac has no Red Heart Containers, he'll respawn with a Half Soul Heart instead.
- The 1up! mushroom begins following Isaac like a familiar upon pickup, but doesn't do anything. It disappears when its life is used up.
- This item belongs to the Fun Guy set. Collecting three items from this set will transform Isaac into a walking mushroom.
- Resurrection items activate in a set order. 1up! is 1st / 2nd in this order after Soul of Lazarus.
- ??? / Tainted ??? / Tainted Bethany / Tainted Forgotten will each respawn with 3 Soul Hearts.
- The Forgotten will respawn with 3 filled Bone Hearts (notably more than his starting total) and The Soul with whatever Soul and Black Hearts he had on death.
- Dying as The Soul respawns him with a Half Soul Heart and The Forgotten with whatever Bone Hearts he had on death.
- Dark Judas / Tainted Judas will respawn with 2 Black Hearts.
PS4 PC CWSQ TNM8 (First Secret Room east of spawn)
- This is a reference to the Super Mario franchise, wherein collecting a green mushroom would give you an extra life.
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