Trisagion

Trisagion is an unlockable passive item added in The Binding of Isaac: Afterbirth †.

Effects

 * Tears are replaced with piercing white blasts of light.
 * Blasts deal 33% of the regular tear damage, but can hit the same enemy multiple times.
 * Blasts decrease in size until disappearing.
 * Despite the blasts decreasing in size after passing through obstacles, this is aesthetic as the blast will only damage enemies adjacent to the obstacle.
 * Blasts do not knock back enemies.

Synergies

 * / Blister / : Adds knockback to blasts, which can not only significantly repel enemies when used correctly, it can deal a high amount of damage to enemies unable to escape the knockback.
 * : Fully charged Trisagion blasts deal 4 times more damage, exceeding Isaac's tear damage.
 * : Constantly shoots tears in cardinal directions during its travel time.
 * : Eyes will fire beams. Occasionally causes Trisagion beams to stay static in the air for a few seconds, dealing damage to enemies that come in contact with them.
 * : Although the damaging aura isn't visible, it still exists and is not affected by Trisagion's damage reduction.
 * Guppy: As it hits multiple times, it spawns multiple flies per tear making it very strong.
 * : Both Trisagion and Haemolacria fire at the same time, Isaac's tears do not keep the spectral attribute despite the sprite being changed. The tears keeps its piercing effect.
 * Homing tears: Tears slow down and stay on top of the enemy, doing continuous damage.
 * : Beams will now travel through obstacles and maintain their piercing effect dealing 33% of regular damage to enemies they come in contact with, but won't explode on hit. The Ipecac explosion will happen once the beam runs out of range or hits a wall and will deal damage equal to Isaac's damage.
 * Keeps Ipecac's stats but shoots a constantly exploding Trisagion beam. There is no way to avoid being hit from the explosion, since the shot immediately explodes. This makes the synergy useless and a disadvantage unless or  is used.
 * : The beam becomes controllable, still dealing reduced damage but at a very high rate.
 * Tiny Planet: Tears circle Isaac skewed towards the direction fired, with high enough tears stat a consistant ring of damaging projectiles can be kept around isaac since Trisagion can hit multiple times.
 * The spore effect can stack multiple times on the same enemy.
 * : In addition to the usual effect, lengthens the Trisagion blasts into short flexible beams for a much larger damage-dealing area.
 * : Causes a large trail of creep. The effect stacks, which causes heavy damage (even to Hush).
 * : Two sideways blasts accompany the beam, effectively increasing horizontal range.
 * Every damage tick will shoot out a Pop tear. Trisagion shots will also hover in the air longer than normal.
 * : Base damage is doubled and beam damage is boosted to about 65%. Blasts lose no power as they travel further, though the tips of the blasts deal very slightly less damage.
 * : The sword will shoot Trisagion beams instead of energy beams, with the Trisagion beams having increased damage.

Interactions

 * Azazel's short-range Brimstone overrides Trisagion.
 * : Overrides Trisagion.
 * : Split blasts are not generated on hitting enemies, only on obstacles and walls.
 * : Split blasts are not generated on hitting enemies, only on obstacles, walls, and the floor.
 * : Overridden by Trisagion.
 * Overrides Trisagion.
 * Overrides Trisagion.
 * : Overridden by Trisagion.
 * : Overridden by Trisagion.
 * : Tears still ignite enemies, but no longer have a chance to explode.
 * : Blasts continually disappear and reappear as they "bounce". The bounces deal 25% splash damage based on Isaac's base rating, but the blasts cannot do their damage while they're disappearing, leading to an overall reduced efficiency.
 * : Overridden by Trisagion.
 * Blasts can apply Holy Light, with a light beam being generated on each damage tick, dealing Isaac's tear damage per beam (three times Trisagion's damage).
 * : Electric arcs are not generated on hitting enemies, only on obstacles and walls.
 * Small electric arcs generate from the blast every tick
 * : The blasts will slow down and float like Lachryphagy tears and eventually burst, but can not consume each other.
 * Blasts have greatly reduced range, but constantly generates Lachryphagy tears, with one shot alone being capable of bursting a tear.
 * : Nearly no effect; increases damage by around 5% when the hit is dealt by the tip of the blast.
 * A Lump of Coal + : As the beam is longer, the amount of extra damage at the tip can be up to around 50%.
 * : Overrides Trisagion.
 * : The baby cannot duplicate Trisagion blasts.
 * Nose Goblin: Overridden by Trisagion.
 * : Creates multiple rifts on the blast's trajectory.
 * : Split blasts are not generated on hitting enemies, only on obstacles and walls.
 * : Overridden by Trisagion.
 * : Rather than applying the effect to each individual tear, all beams will share the same color and change simultaneously every tick.
 * : The blasts will slow down and float like Pop! tears, but will not bounce off each other.
 * : Overridden by Trisagion.
 * : Overrides Trisagion.
 * : Overrides Trisagion.
 * : Unless Isaac has shooting familiars, Technology Zero will not function. Familiars' tears will be connected to the blasts with electricity, however.

Trivia

 * Trisagion was added in Booster Pack, after the initial release of the DLC.
 * Trisagion is based on this Steam Workshop item by x Wyvern x and TRPG, originally known as Lightshot.
 * The name is a reference to one of the oldest Christian hymns.