Dark Room

The Dark Room is one of five final levels in The Binding of Isaac: Rebirth, alongside,  ,  , and. The Dark Room is unlocked by defeating Satan 5 times, after which it can be accessed by touching the chest dropped by Satan with equipped. Entrance to the Dark Room can also be achieved via the I AM ERROR room in, even without , as well as through the Black Market in Sheol, and through a trapdoor spawned by a button. After completing this level or The Chest for the first time, the Dark Room will always contain a large golden door in the first room, which can be unlocked with either the Key Pieces (combination of and ),, , or.

The Dark Room can also be accessed by repeatedly using a Sacrifice Room. After using the spikes for the 12th time, continuing to use the Sacrifice Room will have a 50% chance to teleport Isaac directly to the Dark Room, regardless of what floor he was previously on.

The Dark Room is home to the Grave Room, which will contain a Dirt Patch, which can be dug up using. Doing so for the first time will unlock. On subsequent runs, multiple Dirt Patches can be found, and digging them up using, , or Mom's Shovel will spawn a random type of chest.

Like before it, the Dark Room consists of only one floor. In the Dark Room, it is not possible to bomb doors open to skip rooms. The Dark Room's rooms are mainly filled with bosses and mini-bosses. The boss of the floor is always The Lamb.

The first room the player spawn in will automatically contain 4 Red Chests that function normally or  contain random  Devil Deals. All other types of chests spawned in the Dark Room will always contain Treasure Room items. Destroying Slot Machines, Fortune Telling Machines, and all types of Beggars with the exception of a Shell Game Beggar will also spawn items.

Monsters

 * }

Trivia

 * The Dark Room is the only floor in the game which is original to the initial release of Rebirth. All the other floors included in Rebirth at its launch were from the original The Binding of Isaac and its DLC Wrath of the Lamb.
 * The Dark Room is also the name of a room which, like its name suggests, is plunged into complete darkness, for the notable purpose of processing photos. The fact that access to this floor is granted by touching the chest in while holding, which is the name given to a photo that has not been processed yet and whose colors and hues are inverted, might be a nod to this.
 * The layout to The Chest and the Dark Room are identical for a given seed.
 * One of the Super Secret Room layouts is of the Dark Room and a Black Heart. The sprite for the door is a portal rather than a gray door, however, since bombed-out entrances in the Dark Room appear as portals.
 * This secret room appears floating in a black void on most floors, but in the Dark Room, it appears in a purple misty void instead, just like all the other rooms.
 * One possible room in the Dark Room contains only a Bony and a Lil Haunt. These possibly represent and.
 * On the 6/2/2021 Kickstarter livestream, Edmund McMillen confirmed that the Dark Room represents the closet that Isaac was locked inside in Ending 21.

Music
The music for the Dark Room is titled Devoid, which means “empty”.

Binding of Isaac: Rebirth OST