Darkness Falls

Darkness Falls is challenge #4. is the default character for this challenge, and she starts the challenge with, , , , , and. The goal is to defeat Satan. This Challenge is unlocked once It Lives, and the  are unlocked.

Difficulty
This challenge focuses on a high risk, high reward style of play. When low on health, Eve can inflict more damage. As a result, the majority of the challenge will be spent in a near-death state.

Strategy

 * Since the player is playing as Eve, Whore of Babylon will activate when having 1 Red Heart or less, rather than 1/2. Since she starts with 2 hearts, the Razor Blade can activate this effect immediately.
 * Because the Razor Blade inflicts damage, using it will also activate the Dead Bird.
 * Sacrificial Dagger and Dark Matter can be used together. The fear effect can drive enemies away, allowing Eve to chase and kill them with the dagger.
 * Making one Devil deal can permanently activate Whore of Babylon. However, this can put Eve in a very dangerous position with only one Red Heart. A can produce Soul Hearts, which can allow Eve to maintain a buffer of health while maintaining the Whore of Babylon's effect.
 * Since all of the starting items are from the Devil Room pool, Eve has a much higher chance to get some of the stronger items such as or . As such, opening the Devil Room as often as possible should be a priority.
 * Also, to prevent Angel Rooms from appearing later on in the run, it is advised to take the first Devil Deal encountered.