Eve

Eve is a character unlocked by beating two floors in a row without collecting any heart pickups. Eve starts with two Red Heart containers,, and the.
 * Eve will also start with the upon donating 439 coins to the Greed Donation Machine.
 * However, if the has not been unlocked by defeating Satan with Eve, Eve will not start with the  until both conditions have been fulfilled.

Item Interactions

 * : is active regardless of health and  is active without taking damage.

Good Items

 * Items that take Red Heart containers away typically do so as a cost to counterbalance its potent benefits; for Eve, however, this supposed cost will likely be beneficial due to Whore of Babylon. Such items include, , and.
 * Following the same logic, items that make deals with the Devil more likely will allow Eve to trade her Red Heart containers away more reliably, making it safer to gain health for the sole purpose of spending it in a Devil Room.
 * As Eve will prefer having little to no Red Hearts, she will need alternative ways to stay alive. As such, items that are conducive to spawning Soul Hearts or Black Hearts will be instrumental for Eve. Such items include, , , , and many more.
 * works very well with the, when Eve is at half-a-Red-Heart or less. During the period of invincibility after taking damage, the player can spam the Razor Blade quite a few times and vastly increase Eve's damage with no penalty.
 * Items that activate with low health such as work well with Eve.

Bad Items

 * Any items that give Eve Red Heart containers are potentially bad since it could deactivate the Whore of Babylon effect. However, because Red Heart containers are very useful in deals with the Devil, it could also benefit Eve greatly to hold on to the containers temporarily. Thus, an understanding of Devil Room spawning occurrence and chance is essential in deciding whether or not to take such items.
 * Items that heal Red Heart containers (such as, , and the Tick) can, and oftentimes, inevitably will cancel the effect of Whore of Babylon, if Eve has more than one Red Heart container.
 * Items that attract pick-ups (such as Magneto or Broken Magnet) will force Eve into avoiding Red Hearts so she could keep the Whore of Babylon effect, should she have more than one Red Heart container.
 * Child's Heart causes more Red Hearts to appear, something which is generally undesirable in an Eve run without, and can make more useful items such as bombs or keys less likely to appear.

Trivia

 * Eve was a playable character in the original The Binding of Isaac. She was added with Halloween Update.
 * In the original, Eve was unlocked by making two or more Devil deals in one run. This unlock method was given to Azazel in Rebirth.
 * According to biblical theology, Eve was the first woman on earth, born of Adam's rib in the book of Genesis.
 * In the original game, Eve had a unique effect in that she would leave a bloodstain when entering a room at half a heart remaining instead of a urine stain. This was thought to be a reference to menstruation, as biblically menstruation was a curse upon Eve given by God. This feature was initially (intentionally or unintentionally) left out in Rebirth, but was later re-added in Booster Pack #5.
 * Eve was confirmed to be Edmund McMillen's favorite character from The Binding of Isaac in a Twitter AMA.