Cathedral

The Cathedral is one of two unlockable floors following //, the other being. After defeating Mom's Heart, an Angel Room may spawn containing a beam of light leading upwards to the Cathedral. Unlocking and defeating It Lives will always spawn a beam of light leading to the Cathedral, as well as a trapdoor leading to Sheol.

The Cathedral has only one floor, featuring mostly undead and/or holy enemies, and the boss is always Isaac.

Monsters

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Trivia

 * In the original Binding of Isaac, the windows along the walls were much larger and had different patterns resembling Isaac and a crucifix.
 * One of the interpretations of this floor's theme Everlasting Hymn includes the sentence "Harm yourself and God will grant you strength" many consider this translation "Too extreme" or somehow having no parallels with this game's story, however all the original endings (based on the items Isaac collects) end with Isaac finding several objects and using them to harm himself, which indeed does increase his power in-game

Music
The music for the Cathedral is titled Everlasting Hymn.

Binding of Isaac: Rebirth OST